Anti-E tag 抗体

• ICC

AbReview15783****

Immunocytochemistry - Anti-E tag antibody (AB3397)

ab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 25⁰C. Samples were incubated with primary antibody (1/100 : in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

This image is a courtesy of Anonymous Abreview

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E tag antibody (AB3397)

Immunofluorescent analysis of 4% paraformaldehyde (PFA)- fixed, permeabilized with 0.1%Triton X-100 in CHO cells transfected with 12-tags constructs (CHO-12 tags) labelling E tag with ab3397 at 1/1000 dilution, followed by Donkey Anti-rabbit IgG (H&L), FITC conjugated antibody at 1/1000 dilution (Green) or Donkey Anti-rabbit IgG (H&L), Alexa Fluor594 antibody at 1/500 dilution (Red). Nucleus was counterstained with DAPI (Blue). Images also show parallel staining in parental CHO cell line.