AB212059

Anti-E Cadherin 抗体 [EPR16845-34]

Anti-E Cadherin antibody [EPR16845-34]

RabMAb
Recombinant
Lab Essentials
20ul selling size
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(27 Publications)

Rabbit Recombinant Monoclonal E Cadherin antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 27 publications.

別名を表示する

CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1

3 Images

Supplier Data

Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] (AB212059)

E Cadherin was immunoprecipitated from 1 mg of mouse serum with ab212059 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab212059 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : Mouse serum 10 μg (Input).

Lane 2 : ab212059 IP in mouse serum.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab212059 in mouse serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] (ab212059)

Predicted band size: 97 kDa

false

Supplier Data

Western blot - Anti-E Cadherin antibody [EPR16845-34] (AB212059)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/4/5 : 30 seconds; Lane 2 : 3 minutes; Lane 3 : 15 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 11076937; 11953314).

Lanes 1 - 2:

Western blot - Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/5000 dilution

Lanes 3 - 5:

Western blot - Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/1000 dilution

Lane 1:

Rat spleen lysate at 10 µg

Lane 2:

Rat serum at 10 µg

Lane 3:

Mouse plasma at 10 µg

Lane 4:

Mouse serum at 10 µg

Lane 5:

Mouse brain lysate at 10 µg

Secondary

Lanes 1 and 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 2 - 4:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 120 kDa,84 kDa

false

Supplier Data

Western blot - Anti-E Cadherin antibody [EPR16845-34] (AB212059)

Blocking/Dilution buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/20000 dilution

Lane 2:

Western blot - Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/100000 dilution

All lanes:

Mouse E-Cadherin active protein (aa1-709) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 97 kDa

Observed band size: 120 kDa,84 kDa

false

Exposure time: 10s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16845-34

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity).. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity).. (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.

See full target information Cdh1

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文献 (27)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:417 PubMed40153115

2025

NEK2 promotes cancer cell progression and 5-fluorouracil resistance via the Wnt/β-catenin signaling pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Facai Cui,Yu Chen,Xiaoyu Wu,Weifeng Zhao

Journal of nanobiotechnology 23:150 PubMed40016753

2025

A self-adjuvant multiantigenic nanovaccines simultaneously activate the antiviral and antitumor immunity for the treatment of cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongjie Wang,Hanlin Chen,Ruiqi Ming,Weiwei Wang,Shujun Liu,Yuantian Jing,Zewei Yan,Guihong Lu,Li-Li Huang

World journal of gastrointestinal oncology 17:96230 PubMed39958556

2025

LncRNA PCAT6 promotes progression and metastasis of colonic neuroendocrine carcinoma MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Wang,Hai-Feng Mu,Chun Wang,Yue Tang,Ming-Yuan Si,Jing Peng

The Kaohsiung journal of medical sciences 40:699-709 PubMed39031804

2024

circUQCRC2 promotes asthma progression in children by activating the VEGFA/NF-κB pathway by targeting miR-381-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Juan Yang,Shu-Xiang Sui,Qing-Hua Zheng,Min Wang

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 75:205-213 PubMed38736267

2024

Campothecin suppresses cell proliferation and migration in head and neck squamous cell carcinoma by blocking RAB27A-mediated phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Y Zhao,Y Wang,L Zhao,L Qu,J H Zheng

Cellular signalling 117:111087 PubMed38316266

2024

FATP2 activates PI3K/Akt/mTOR pathway by inhibiting ATF3 and promotes the occurrence and development of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Jiang,Ru Chen,Bing Hu,Situ Xiong,Sheng Li,Bin Fu,Xiaoqiang Liu

International journal of chronic obstructive pulmonary disease 19:33-50 PubMed38197032

2024

Abhd2, a Candidate Gene Regulating Airway Remodeling in COPD via TGF-β.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Yu Lv,Ling-Ling Jin,Xi-Qiao Sang,Wen-Chao Shi,Li-Xia Qiang,Qing-Yan Lin,Shou-De Jin

Clinical ophthalmology (Auckland, N.Z.) 17:3047-3056 PubMed37869043

2023

Participates in Anti-VEGF-Induced Epithelial Mesenchymal Transformation in RPE Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xianghui Hao,Yingbin Hua,Chaohui Xie,Haifeng Xu

International journal of molecular sciences 24: PubMed37445879

2023

Skin Wound following Irradiation Aggravates Radiation-Induced Brain Injury in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Mang Xiao,Xianghong Li,Li Wang,Bin Lin,Min Zhai,Lisa Hull,Alex Zizzo,Wanchang Cui,Juliann G Kiang

Biomolecules & therapeutics 31:434-445 PubMed37019836

2023

Effect of FTY-720 on Pulmonary Fibrosis in Mice via the TGF-β1 Signaling Pathway and Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Jin,Weidong Liu,Ge Gao,Yilan Song,Hanye Liu,Liangchang Li,Jiaxu Zhou,Guanghai Yan,Hong Cui

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E Cadherin 抗体 [EPR16845-34]

Anti-E Cadherin antibody [EPR16845-34]

Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] (AB212059)

Western blot - Anti-E Cadherin antibody [EPR16845-34] (AB212059)

Western blot - Anti-E Cadherin antibody [EPR16845-34] (AB212059)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (27)

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Abcam product promise