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リコンビナント

Anti-E Cadherin 抗体 [36/E-Cadherin] - BSA and Azide free (ab287971)

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Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
  • Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
  • Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
  • Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [36/E-Cadherin] to E Cadherin - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free
    E Cadherin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [36/E-Cadherin] to E Cadherin - BSA and Azide free

  • 由来種

    Mouse

  • 特異性

    This antibody recognises overexpressed P-cadherin and may have some degree of cross-reactivity with endogenous P-caderin

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T transfected with E-Cadherin expression vector containing a myc-His-tag®, whole cell lysate. MCF7 whole cell lysate. ICC/IF: MCF7 cells.

  • 特記事項

    This antibody is a carrier free version of ab287970. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab287971の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

  • 組織特異性

    Non-neural epithelial tissues.

  • 関連疾患

    Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.

  • 配列類似性

    Contains 5 cadherin domains.

  • 翻訳後修飾

    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.

  • 細胞内局在

    Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Arc 1 antibody
      • CADH1_HUMAN antibody
      • Cadherin 1 antibody
      • cadherin 1 type 1 E-cadherin antibody
      • Cadherin-1 antibody
      • Cadherin1 antibody
      • CAM 120/80 antibody
      • CD 324 antibody
      • CD324 antibody
      • CD324 antigen antibody
      • cdh1 antibody
      • CDHE antibody
      • E-Cad/CTF3 antibody
      • E-cadherin antibody
      • ECAD antibody
      • Epithelial cadherin antibody
      • epithelial calcium dependant adhesion protein antibody
      • LCAM antibody
      • Liver cell adhesion molecule antibody
      • UVO antibody
      • Uvomorulin antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
      Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)

      This data was developed using ab287970, the same antibody clone in a different buffer formulation. 

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) whole cell lysate labeling E Cadherin with ab287970 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in MCF7 cell line. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab179513) at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab287970 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab179513) at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
      Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
      All lanes : Anti-E Cadherin antibody [36/E-Cadherin] (ab287970) at 1/1000 dilution

      Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 97 kDa
      Additional bands at: 120, 110 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 7 seconds


      This data was developed using ab287970, the same antibody clone in a different buffer formulation. 

      5% NFDM/TBST was used as blocking and diluting buffer. 

      Full-length E-cadherin is ~120 kDa. The other bands are due to proteolytic cleavage in different Cadherin domains (PMID: 11112695; PMID: 11212238).

    • Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
      Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
      All lanes : Anti-E Cadherin antibody [36/E-Cadherin] (ab287970) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T transfected with P-Cadherin expression vector containing a myc-His-tag®, whole cell lysate
      Lane 3 : HEK-293T transfected with E-Cadherin expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 97 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      This data was developed using ab287970, the same antibody clone in a different buffer formulation. 

      5% NFDM/TBST was used as blocking and diluting buffer. 

      This antibody has cross-reactivity with P-Cadherin.

    • Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)
      Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab287971 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab287971 は論文での使用が確認できていません。

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