AB287970

Anti-E Cadherin 抗体 [36/E-Cadherin]

Anti-E Cadherin antibody [36/E-Cadherin]

Recombinant
Lab Essentials
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(3 Publications)

Mouse Recombinant Monoclonal E Cadherin antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 3 publications.

別名を表示する

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] (AB287970)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] (AB287970)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) whole cell lysate labeling E Cadherin with ab287970 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150117) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in MCF7 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab179513) at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab287970 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/500 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab179513) at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150117) secondary antibody at 1/1000 dilution.

Supplier Data

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (AB287970)

5% NFDM/TBST was used as blocking and diluting buffer.

Full-length E-cadherin is ~120 kDa. The other bands are due to proteolytic cleavage in different Cadherin domains (PMID : 11112695; PMID : 11212238).

All lanes:

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (ab287970) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 110 kDa,120 kDa

false

Exposure time: 7s

Supplier Data

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (AB287970)

5% NFDM/TBST was used as blocking and diluting buffer.

This antibody has cross-reactivity with P-Cadherin.

All lanes:

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (ab287970) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with P-Cadherin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with E-Cadherin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 120 kDa

false

Exposure time: 3s

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

36/E-Cadherin

アイソタイプ

IgG2a

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody recognises overexpressed P-cadherin and may have some degree of cross-reactivity with endogenous P-caderin

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.

See full target information CDH1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Medicine international 5:14 PubMed39790708

2025

Prognostic factors for first recurrence following meningioma surgery.

Applications

Unspecified application

Species

Unspecified reactive species

George Fotakopoulos,Vasiliki Epameinondas Georgakopoulou,Demetrios A Spandidos,Efthalia Angelopoulou,Apostolos-Alkiviadis Menis,Nikolaos Trakas,George Alexiou,Spyridon Voulgaris

Journal of advanced research 60:215-231 PubMed37479180

2023

Procyanidin C1 inhibits tumor growth and metastasis in colon cancer via modulating miR-501-3p/HIGD1A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Lin Lv,Yu-Jun Tan,Yu-Shan Ren,Ru Ma,Xiao Wang,Shu-Yan Wang,Wan-Qing Liu,Qiu-Sheng Zheng,Jing-Chun Yao,Jun Tian,Jie Li

Cytotechnology 74:657-667 PubMed36389286

2022

Scaffold-based three-dimensional cell model of pancreatic cancer is more suitable than scaffold-free three-dimensional cell model of pancreatic cancer for drug discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Dafei Xie,Shengnan Jia,Dongnan Ping,Dong Wang,Liping Cao

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E Cadherin 抗体 [36/E-Cadherin]

Anti-E Cadherin antibody [36/E-Cadherin]

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] (AB287970)

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (AB287970)

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (AB287970)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise