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AB7164

Anti-Dystrophin 抗体 [MANDRA1]

Anti-Dystrophin antibody [MANDRA1]

5

(1 Review)

|

(24 Publications)

Anti-Dystrophin antibody [MANDRA1] (ab7164) is a mouse monoclonal antibody detecting Dystrophin in Western Blot, IHC-Fr, ICC/IF, ELISA. Suitable for Human.

- Over 20 publications
- Trusted since 2001

別名を表示する

Dystrophin, DMD

1 Images
Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [MANDRA1] (AB7164)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [MANDRA1] (AB7164)

ab7164 staining Dystrophin in frozen human tongue tissue sections by Immunohistochemistry (IHC - Fr- Frozen sections). Samples were incubated 1 : 100 dilution. A Goat Anti-mouse, FITC- conjugate was used as the secondary antibody.

This image was generated using the ascites version of the product.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MANDRA1

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

ELISA, IHC-Fr, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human DMD aa 3200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11532

エピトープ

128 amino acids at the end of the C-terminal domain of the human dystrophin molecule (a.a. residues 3558-3684).

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Reactivity data

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製品の詳細

What is this antibody validated in?
Anti-Dystrophin antibody [MANDRA1] (ab7164) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human samples.

Trusted by the scientific community
Anti-Dystrophin [MANDRA1] (ab7164) was first used in a scientific publication in 2001 and has been cited over 20 times in peer-reviewed journals.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Proprietary technique
精製に関する特記事項
Purified from culture supernatant of hybridoma cells with proprietary method.
バッファー組成
Preservative: 0.0975% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Dystrophin also known as the DMD protein plays a mechanical role in muscle fibers by connecting the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. This structural connection helps reinforce the muscle fiber during contraction and mechanical stress. The protein has a molecular weight of approximately 427 kDa. It is expressed mainly in skeletal and cardiac muscles where it is important for maintaining muscle integrity.
Biological function summary

Dystrophin acts as an important component of the dystrophin-glycoprotein complex. This complex stabilizes the muscle cell membrane by linking actin filaments within the cytoskeleton to proteins in the extracellular matrix. The absence or malfunctioning of dystrophin disrupts this connection leading to increased susceptibility to damage during muscle contraction. This is especially evident in tissues where the protein is abundantly present.

Pathways

Dystrophin is integral to the structural integrity pathway in muscle cells. It works alongside proteins like dystroglycan and sarcoglycan forming a multiprotein complex that ensures cell membrane stability during muscle contractions. The proper functioning of the dystrophin complex is also linked to calcium signaling pathways highlighting its role in cellular signaling mechanisms.

Dystrophin's malfunction is directly associated with Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Mutations in the DMD gene which encodes the dystrophin protein result in the absence or reduced functionality of the protein leading to progressive muscle degeneration observed in DMD and BMD. These disorders frequently involve the protein utrophin which sometimes compensates for the lack of functional dystrophin albeit insufficiently to alleviate the symptoms.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
See full target information DMD
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文献 (24)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:18984 PubMed40447687

2025

Neuronal hyperexcitability in dystrophin-deficient mdx hippocampal neurons: the importance of interleukin-6 and GABAergic regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberley A Stephenson,Qiao Xiao,Michael B Vaughan,Polly Peters,Claire Denley,Sean O'Meara,Cecelia Sheehan,Mark G Rae,Dervla O'Malley

International journal of molecular sciences 23: PubMed36233175

2022

Dp71 Point Mutations Induce Protein Aggregation, Loss of Nuclear Lamina Integrity and Impaired Braf35 and Ibraf Function in Neuronal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Ivette Rugerio-Martínez,Daniel Ramos,Abel Segura-Olvera,Nadia Mireya Murillo-Melo,Yessica Sarai Tapia-Guerrero,Raúl Argüello-García,Norberto Leyva-García,Oscar Hernández-Hernández,Bulmaro Cisneros,Rocío Suárez-Sánchez

Phytotherapy research : PTR 36:3265-3275 PubMed35606908

2022

Resveratrol ameliorates muscle atrophy in chronic kidney disease via the axis of SIRT1/FoxO1.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiting Wang,Weidong Yuan,Lu Li,Fei Lu,Lingling Zhang,Haifeng Gong,Xinzhong Huang

Glia 70:466-490 PubMed34773297

2021

Dystrophin deficiency affects human astrocyte properties and response to damage.

Applications

Unspecified application

Species

Unspecified reactive species

Jenny Lange,Olivia Gillham,Reem Alkharji,Simon Eaton,Giulia Ferrari,Monika Madej,Michael Flower,Francesco Saverio Tedesco,Francesco Muntoni,Patrizia Ferretti

Eye and vision (London, England) 8:13 PubMed33865457

2021

Anti-VEGF therapy prevents Müller intracellular edema by decreasing VEGF-A in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Tianqin Wang,Chaoyang Zhang,Hai Xie,Mengmeng Jiang,Haibin Tian,Lixia Lu,Guo-Tong Xu,Lin Liu,Jingfa Zhang

Scientific reports 10:17621 PubMed33077830

2020

Cytoskeletal disorganization underlies PABPN1-mediated myogenic disability.

Applications

Unspecified application

Species

Unspecified reactive species

Cyriel Sebastiaan Olie,Erik van der Wal,Cikes Domagoj,Loes Maton,Jessica C de Greef,I-Hsuan Lin,Yi-Fan Chen,Elsayad Kareem,Josef M Penninger,Benedikt M Kessler,Vered Raz

eLife 8: PubMed31829939

2019

Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Akiyoshi Uezu,Erin Hisey,Yoshihiko Kobayashi,Yudong Gao,Tyler Wa Bradshaw,Patrick Devlin,Ramona Rodriguiz,Purushothama Rao Tata,Scott Soderling

Stem cells (Dayton, Ohio) 37:1615-1628 PubMed31574188

2019

CRISPR/Cas9-Based Dystrophin Restoration Reveals a Novel Role for Dystrophin in Bioenergetics and Stress Resistance of Muscle Progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Polina R Matre,Xiaodong Mu,Jianbo Wu,Delia Danila,Mary A Hall,Mikhail G Kolonin,Radbod Darabi,Johnny Huard

Stem cells (Dayton, Ohio) 35:725-738 PubMed27612437

2016

Dynamic Phosphorylation of the Myocyte Enhancer Factor 2Cα1 Splice Variant Promotes Skeletal Muscle Regeneration and Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Fiorenza Baruffaldi,Didier Montarras,Valentina Basile,Luca De Feo,Sara Badodi,Massimo Ganassi,Renata Battini,Carmine Nicoletti,Carol Imbriano,Antonio Musarò,Susanna Molinari

The Journal of general physiology 147:395-406 PubMed27069119

2016

Functional characterization of orbicularis oculi and extraocular muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Marijana Sekulic-Jablanovic,Nina D Ullrich,David Goldblum,Anja Palmowski-Wolfe,Francesco Zorzato,Susan Treves
View all publications
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