Anti-Dyrk1B/MIRK 抗体 (ab113968)
Key features and details
- Rabbit polyclonal to Dyrk1B/MIRK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Dyrk1B/MIRK antibody -
製品の詳細
Rabbit polyclonal to Dyrk1B/MIRK -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide corresponding to Human Dyrk1B/MIRK aa 500-550.
Database link: NP_004705.1 -
ポジティブ･コントロール
- HeLa whole cell lysate (ab150035)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113968の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
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| IP |
Use at 5-15 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 69 kDa. |
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IP
Use at 5-15 µg/mg of lysate. |
ターゲット情報
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機能
Dual-specificity kinase which possesses both serine/ threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. -
組織特異性
Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 507571 Cow
- Entrez Gene: 9149 Human
- Entrez Gene: 13549 Mouse
- Entrez Gene: 308468 Rat
- Entrez Gene: 698848 Rhesus monkey
- Omim: 604556 Human
- SwissProt: Q9Y463 Human
- SwissProt: Q9Z188 Mouse
see all -
別名
- AOMS3 antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1B antibody
see all
画像
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Western blot - Anti-Dyrk1B/MIRK antibody (ab113968)All lanes : Anti-Dyrk1B/MIRK antibody (ab113968) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 1 minute -
Immunoprecipitation - Anti-Dyrk1B/MIRK antibody (ab113968)Detection of Dyrk1B/MIRK in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113968 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113968 は論文での使用が確認できていません。