Anti-DYRK1A 抗体 (ab65220)
Key features and details
- Rabbit polyclonal to DYRK1A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DYRK1A antibody
DYRK1A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DYRK1A -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human DYRK1A aa 21-70 (internal sequence).
Database link: Q13627 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab65220 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65220の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
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| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
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| 特記事項 |
|---|
|
ICC/IF
1/500 - 1/1000. |
|
WB
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
ターゲット情報
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機能
May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates. -
組織特異性
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney. -
関連疾患
Defects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
発生段階
Expressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain. -
ドメイン
The polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028). -
翻訳後修飾
Autophosphorylated on tyrosine residues. -
細胞内局在
Nucleus speckle. - Information by UniProt
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参照データベース
- Entrez Gene: 1859 Human
- Omim: 600855 Human
- SwissProt: Q13627 Human
- Unigene: 368240 Human
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別名
- Dual specificity tyrosine phosphorylation regulated kinase antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-DYRK1A antibody (ab65220)ab65220, at a 1/500 dilution, staining DYRK1A in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Western blot - Anti-DYRK1A antibody (ab65220)All lanes : Anti-DYRK1A antibody (ab65220) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide at 10 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 86 kDa
Observed band size: 86 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab65220 は 3 報の論文で使用されています。
- Fernandez Bessone I et al. DYRK1A Regulates the Bidirectional Axonal Transport of APP in Human-Derived Neurons. J Neurosci 42:6344-6358 (2022). PubMed: 35803734
- Fujita K et al. Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo. Int J Oncol 46:2419-30 (2015). PubMed: 25823465
- Baek KH et al. Down's syndrome suppression of tumour growth and the role of the calcineurin inhibitor DSCR1. Nature 459:1126-30 (2009). WB ; Human . PubMed: 19458618