Anti-Dynamin 2 抗体

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (AB3457)

Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa cells labeling Dynamin 2 with ab3457 at 2 ug/ml followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green).

The nuclear counterstain is DAPI.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (AB3457)

ab3457 (1µg/ml) staining Dynamin 2 in human lung using an automated system (DAKO Autostainer Plus).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (AB3457)

ICC/IF image of ab3457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (AB3457)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y cells labeling Dynamin 2 with ab3457 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green). The nuclear counterstain is DAPI (blue), and F-actin is stained with Rhodamine Phalloidin iat 1/300 dilution (red). Negative control was cells without primary antibody present.

• WB

Supplier Data

Western blot - Anti-Dynamin 2 antibody (AB3457)

All lanes:

Western blot - Anti-Dynamin 2 antibody (ab3457)

Lane 1:

SH-SY5Y whole cell lysate

Lane 2:

PC-12

Lane 3:

Neuro-2a

Lane 4:

SK-N-AS

Lane 5:

U- 87 MG

Lane 6:

Rat Brain

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugated

Predicted band size: 98 kDa

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