DyLight® 650 Anti-6X His tag® 抗体 [AD1.1.10] (ab117504)
![Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)](https://www.abcam.co.jp/ps/products/117/ab117504/Images/ab117504-276742-anti-6x-his-tag-antibody-ad1110-dylight-650-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)")
Key features and details
- DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]
6X His tag® 一次抗体 製品一覧 -
製品の詳細
DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag® -
由来種
Mouse -
標識
DyLight® 650. Ex: 654nm, Em: 673nm -
特異性
ab117504 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.
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アプリケーション
適用あり: ICC/IF, Flow Cytmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresonding to 6X His tag®.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
バッファー
pH: 8.20
Constituent: 99% Tris buffered saline -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
AD1.1.10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- Anti-6X His tag® antibody [AD1.1.10] (ab15149)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117504の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
1/500 - 1/5000.
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
1/500 - 1/5000. |
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Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells transfected with His-tagged actin labeling 6X His tag® with ab117504 at 1/1000 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in PBS. The cells were blocked with 5% BSA for 12 hours at 4°C, followed by incubation with Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) for 2 hours at 25°C.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab117504 は 8 報の論文で使用されています。
- Zhang J et al. Reduction of pl-CSA through ChSy-2 knockout inhibits tumorigenesis and metastasis of choriocarcinoma in JEG3 cells. Int J Med Sci 18:207-215 (2021). PubMed: 33390789
- Gao H et al. Nuclear imaging-guided PD-L1 blockade therapy increases effectiveness of cancer immunotherapy. J Immunother Cancer 8:N/A (2020). PubMed: 33203663
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
- Nguyen HQ et al. Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads. Elife 8:N/A (2019). PubMed: 31282865
- Harink B et al. An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs). PLoS One 14:e0203725 (2019). PubMed: 30901328
- Natarajan A et al. A Novel Engineered Small Protein for Positron Emission Tomography Imaging of Human Programmed Death Ligand-1: Validation in Mouse Models and Human Cancer Tissues. Clin Cancer Res 25:1774-1785 (2019). PubMed: 30373750
- Imhof BA et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). PubMed: 27482114
- Tillotson BJ et al. Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation. PLoS One 10:e0145820 (2015). PubMed: 26713870
