AB181262

Anti-DUS2L 抗体 [EPR14042]

Anti-DUS2L antibody [EPR14042]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal DUS2L antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

別名を表示する

DUS2L, DUS2, tRNA-dihydrouridine(20) synthase [NAD(P)+]-like, Dihydrouridine synthase 2, Up-regulated in lung cancer protein 8, tRNA-dihydrouridine synthase 2-like, URLC8, hDUS2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-DUS2L antibody [EPR14042] (AB181262)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DUS2L antibody [EPR14042] (AB181262)

Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling DUS2L with ab181262 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Flow Cytometry (Intracellular) - Anti-DUS2L antibody [EPR14042] (AB181262)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DUS2L antibody [EPR14042] (AB181262)

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling DUS2L with ab181262 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] (AB181262)

Western blot analysis of immunoprecipitation pellet from A549 cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181262 at 1/50 dilution.
Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] (ab181262)

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-DUS2L antibody [EPR14042] (AB181262)

All lanes:

Western blot - Anti-DUS2L antibody [EPR14042] (ab181262) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14042

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra), WB, IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (PubMed : 15994936, PubMed : 26429968, PubMed : 30149704, PubMed : 34798057, PubMed : 38680565). Specifically modifies U20 in cytoplasmic tRNAs (PubMed : 38680565). Activity depends on the presence of guanosine at position 19 in the tRNA substrate (PubMed : 38680565). Negatively regulates the activation of EIF2AK2/PKR (PubMed : 18096616).

See full target information DUS2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 18:4239-4246 PubMed30221720

2018

Long non‑coding RNA ferritin heavy polypeptide 1 pseudogene 3 controls glioma cell proliferation and apoptosis via regulation of the microRNA‑224‑5p/tumor protein D52 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Zhang,Ying Li,Jing Wang,Ping Lei

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DUS2L 抗体 [EPR14042]

Anti-DUS2L antibody [EPR14042]

Immunocytochemistry/ Immunofluorescence - Anti-DUS2L antibody [EPR14042] (AB181262)

Flow Cytometry (Intracellular) - Anti-DUS2L antibody [EPR14042] (AB181262)

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] (AB181262)

Western blot - Anti-DUS2L antibody [EPR14042] (AB181262)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise