AB176727

Anti-DUBA 抗体

Name: Anti-DUBA 抗体 (ab176727) | Abcam
Brand: Abcam Limited
SKU: AB176727
Price: 76000 JPY
Availability: InStock

Anti-DUBA antibody

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(5 Publications)

Rabbit Polyclonal DUBA antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human OTUD5 aa 150-500.

別名を表示する

OTU domain-containing protein 5, Deubiquitinating enzyme A, DUBA, OTUD5

2 Images

Supplier Data

Immunoprecipitation - Anti-DUBA antibody (AB176727)

Western blot of immunoprecipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling DUBA using ab176727 used for WB at 1 μg/ml and used for IP at 3 μg/mg lysate, using chemiluminescence with exposure times of 10 seconds.

All lanes:

Immunoprecipitation - Anti-DUBA antibody (ab176727)

Predicted band size: 61 kDa

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Supplier Data

Western blot - Anti-DUBA antibody (AB176727)

Chemiluminescence with exposure times of 3 minutes.

All lanes:

Western blot - Anti-DUBA antibody (ab176727) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 61 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human OTUD5 aa 150-500. The exact immunogen used to generate this antibody is proprietary information.

Q96G74

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Deubiquitinating enzyme that functions as a negative regulator of the innate immune system (PubMed : 17991829, PubMed : 22245969, PubMed : 23827681, PubMed : 33523931). Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 22245969). Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (PubMed : 22245969). Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production (PubMed : 17991829). Controls neuroectodermal differentiation through cleaving 'Lys-48'-linked ubiquitin chains to counteract degradation of select chromatin regulators such as ARID1A, HDAC2 and HCF1 (PubMed : 33523931). Acts as a positive regulator of mTORC1 and mTORC2 signaling following phosphorylation by MTOR : acts by mediating deubiquitination of BTRC, leading to its stability (PubMed : 33110214).

See full target information OTUD5

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406700 PubMed39721018

2024

OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Song,Tengfei Liu,Lu Yu,Qiuran Ji,Xin Guo,Runzhe Zong,Yiquan Li,Gan Huang,Qidi Xue,Qingyi Fu,Bingyu Liu,Yi Zheng,Lin Chen,Chengjiang Gao,Huiqing Liu

Cell death and differentiation 31:753-767 PubMed38605168

2024

OTUD5 promotes the inflammatory immune response by enhancing MyD88 oligomerization and Myddosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxing Liu,Jiahua Yuan,Yuling Zhang,Fei Qin,Xuemei Bai,Wanwei Sun,Tian Chen,Feng Liu,Yi Zheng,Xiaopeng Qi,Wei Zhao,Bingyu Liu,Chengjiang Gao

Bosnian journal of basic medical sciences 22:901-911 PubMed35765958

2022

Mechanism of OTUD5 in non-small cell lung cancer cell proliferation, invasion, and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Xuebing Li,Baohua Lu,Lina Zhang,Jing Yang,Yurong Cheng,Dong Yan

Cell death and differentiation 28:900-914 PubMed33110214

2020

Deubiquitinase OTUD5 is a positive regulator of mTORC1 and mTORC2 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hwa Cho,Kidae Kim,Sung Ah Kim,Sungryul Park,Bi-Oh Park,Jong-Hwan Kim,Seon-Young Kim,Min Jee Kwon,Myeong Hoon Han,Sung Bae Lee,Byoung Chul Park,Sung Goo Park,Jeong-Hoon Kim,Sunhong Kim

The Journal of biological chemistry 292:11804-11814 PubMed28559278

2017

The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hwa Cho,Sung Ah Kim,Yeon-Soo Seo,Sung Goo Park,Byoung Chul Park,Jeong-Hoon Kim,Sunhong Kim

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DUBA 抗体

Anti-DUBA antibody

Immunoprecipitation - Anti-DUBA antibody (AB176727)

Western blot - Anti-DUBA antibody (AB176727)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (5)

代替製品

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組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise