Dual color: FITC + PE Anti-CD4 + CD8 抗体 [EDU-2 + 733] (ab1232)
Key features and details
- Dual color: FITC + PE Mouse monoclonal [EDU-2 + 733] to CD4 + CD8
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Dual color: FITC + PE
製品の概要
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製品名
Dual color: FITC + PE Anti-CD4 + CD8 antibody [EDU-2 + 733]
CD4 + CD8 一次抗体 製品一覧 -
製品の詳細
Dual color: FITC + PE Mouse monoclonal [EDU-2 + 733] to CD4 + CD8 -
由来種
Mouse -
標識
Dual color: FITC + PE -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. Stimulated human leukocytes
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特記事項
This cocktail is mix of: CD4 = Clone:EDU-2, Isotype:IgG2a, Labeled with FITC and CD8 = Clone:733, Isotype:IgG1, Labeled with PE.This product contains sodium azide, which under acid conditions yields hydrazoic acid, a toxic compound. Azide compounds should be diluted with running water before being discarded to avoid deposits in lead or copper plumbing where explosive conditions may develop.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
EDU-2 + 733 -
ミエローマ
unknown -
軽鎖の種類
unknown -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1232の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
| 特記事項 |
|---|
Percentage count of T suppressor/cytotoxic lymphocytes (CD8+).
Control autoimmune pathologies, immunodeficiencies and immunosuppressivetherapies.
Studies of HIV (Human Immunodeficiency Virus) infection.
CD4(FITC) / CD8 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD4(FITC) / CD8 (PE) is sufficient for labelling 1x106 cells.
ターゲット情報
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関連性
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. -
細胞内局在
Membrane; single pass type I membrane protein. -
参照データベース
- Entrez Gene: 920 Human
- Entrez Gene: 925 Human
- Entrez Gene: 926 Human
- Omim: 186730 Human
- Omim: 186910 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- SwissProt: P01732 Human
see all -
別名
- CD4 antigen antibody
- CD4 molecule antibody
- CD4 mut antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab1232 は 2 報の論文で使用されています。
- Xu W et al. Tet1 Regulates Astrocyte Development and Cognition of Mice Through Modulating GluA1. Front Cell Dev Biol 9:644375 (2021). PubMed: 34778243
- Wang D et al. Genome-wide DNA methylation pattern in a mouse model reveals two novel genes associated with Staphylococcus aureus mastitis. Asian-Australas J Anim Sci 33:203-211 (2020). PubMed: 31010979