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リコンビナントRabMAb

Anti-DSTN 抗体 [EPR15827(B)] (ab186754)

Reviews (1) Submit a question References (5)
Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Flow Cytometry (Intracellular) - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Immunoprecipitation - Anti-DSTN antibody [EPR15827(B)] (ab186754)
  • Anti-DSTN antibody [EPR15827(B)] (ab186754)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15827(B)] to DSTN
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-DSTN antibody [EPR15827(B)]
    DSTN 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15827(B)] to DSTN

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab128429)

  • ポジティブ･コントロール

    • Recombinant Human DSTN protein (ab128429) can be used as a positive control in WB. MCF-7, HeLa, A549, C6, PC-12 and NIH/3T3 cell lysates; Human bladder transitional cell carcinoma tissue; HeLa and MCF-7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR15827(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186754の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/360.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500 - 1/1000.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).Can be blocked with Recombinant Human DSTN protein (ab128429).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30 - 1/50.
特記事項
Flow Cyt (Intra)
1/360.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500 - 1/1000.
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).Can be blocked with Recombinant Human DSTN protein (ab128429).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30 - 1/50.

ターゲット情報

画像

  • Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/10000 dilution + MCF-7 cell lyate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    ab186754 staining DSTN in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol.. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

  • Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    All lanes : Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    All lanes : Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling DSTN with ab186754 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling DSTN with ab186754 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF-7 cells labeling DSTN with ab186754 at 1/1000 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Flow Cytometry (Intracellular) - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling DSTN with ab186754 at 1/360 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. 

     

     

  • Immunoprecipitation - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunoprecipitation - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Western blot analysis of DSTN in A549 cell lysate immunoprecipitated with ab186754 at 1/50 dilution. 

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Anti-DSTN antibody [EPR15827(B)] (ab186754)

参考文献 (5)

ab186754 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab186754 は 5 報の論文で使用されています。

  • Li C  et al. Glutaredoxin 1 protects lens epithelial cells from epithelial-mesenchymal transition by preventing casein kinase 1α S-glutathionylation during posterior capsular opacification. Redox Biol 62:102676 (2023). PubMed: 36989576
  • Wen R  et al. DSTN Hypomethylation Promotes Radiotherapy Resistance of Rectal Cancer by Activating the Wnt/β-Catenin Signaling Pathway. Int J Radiat Oncol Biol Phys 117:198-210 (2023). PubMed: 37019366
  • Moore AS  et al. Actin cables and comet tails organize mitochondrial networks in mitosis. Nature 591:659-664 (2021). PubMed: 33658713
  • Gong L  et al. WDHD1 Leads to Cisplatin Resistance by Promoting MAPRE2 Ubiquitination in Lung Adenocarcinoma. Front Oncol 10:461 (2020). PubMed: 32426268
  • Swärd K  et al. Identification of the intermediate filament protein synemin/SYNM as a target of myocardin family coactivators. Am J Physiol Cell Physiol 317:C1128-C1142 (2019). PubMed: 31461342

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Western blot abreview for Anti-DSTN antibody [EPR15827(B)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SAOS cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
SAOS cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Sep 12 2017

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