Anti-ds DNA 抗体 [35I9 DNA] - BSA and Azide free (ab27156)
Key features and details
- Mouse monoclonal [35I9 DNA] to ds DNA - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free
ds DNA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [35I9 DNA] to ds DNA - BSA and Azide free -
由来種
Mouse -
特異性
Primarily Double stranded DNA. Measurements by immuno-CE yielded KD's of 0.71 µM and 0.09 µM, for the interaction of this antibody with ss- and dsDNA, respectively. Strong reactivity with both ss- and dsDNA has been observed on dotblots as well as very weak reactivity with RNA. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
The details of the immunogen for this antibody are not available.
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ポジティブ・コントロール
- IHC-P: human skin tissue sections.
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特記事項
This antibody has been shown to be useful, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength.
Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113). Or search our wide range of secondary antibodies for use with your experiment.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
This antibody has been shown to be useful, using immunofluorescence, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength. -
ポリ/モノ
モノクローナル -
クローン名
35I9 DNA -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27156の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs. -
細胞内局在
Nuclear -
別名
- Double stranded deoxyribonucleic acid antibody
- Double stranded DNA antibody
- dsDNA antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156)
IHC image of ds DNA staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab27156, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (141)
ab27156 は 141 報の論文で使用されています。
- Karam J et al. Rapid communication: insights into the role of extracellular vesicles during Auger radioimmunotherapy. Int J Radiat Biol 99:109-118 (2023). PubMed: 34270378
- Shen H et al. Synergistically targeting synovium STING pathway for rheumatoid arthritis treatment. Bioact Mater 24:37-53 (2023). PubMed: 36582350
- Chung SY et al. A G-quadruplex stabilizer, CX-5461 combined with two immune checkpoint inhibitors enhances in vivo therapeutic efficacy by increasing PD-L1 expression in colorectal cancer. Neoplasia 35:100856 (2023). PubMed: 36442297
- Nassour J et al. Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis. Nature 614:767-773 (2023). PubMed: 36755096
- Smolka JA & Lewis SC In Situ Analysis of Mitochondrial DNA Synthesis Using Metabolic Labeling Coupled to Fluorescence Microscopy. Methods Mol Biol 2615:99-106 (2023). PubMed: 36807787