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リコンビナントRabMAb

Anti-DRP1 抗体 [EPR19275] (ab184248)

Submit a review Submit a question References (8)
Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Flow Cytometry (Intracellular) - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Immunoprecipitation - Anti-DRP1 antibody [EPR19275] (ab184248)
  • Anti-DRP1 antibody [EPR19275] (ab184248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19275] to DRP1
  • Suitable for: IP, IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-DRP1 antibody [EPR19275]
    DRP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19275] to DRP1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, IHC-P, WB, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human fetal heart and fetal kidney lysates; A549, HeLa, Jurkat, HEK-293, HCT 116, HepG2, PC-12, NIH/3T3 and U-2 OS whole cell lysates; Mouse and rat brain, heart and spleen lysates. IHC-P: Human cerebellum and glioma tissues; Mouse and rat cerebellum tissues. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184248の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/30.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Flow Cyt (Intra)
1/500.
特記事項
IP
1/30.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Flow Cyt (Intra)
1/500.

ターゲット情報

  • 機能

    Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
    Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.

  • 組織特異性

    Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.

  • 関連疾患

    Note=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.

  • 配列類似性

    Belongs to the dynamin family.
    Contains 1 GED domain.

  • ドメイン

    The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.

  • 翻訳後修飾

    Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
    Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
    S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
    Ubiquitination by MARCH5 affects mitochondrial morphology.

  • 細胞内局在

    Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DLP1 antibody
    • dnm1l antibody
    • DNM1L_HUMAN antibody
    • Dnm1p/Vps1p-like protein antibody
    • dnml1 antibody
    • DRP1 antibody
    • DVLP antibody
    • Dymple antibody
    • Dynamin 1 like antibody
    • Dynamin family member proline-rich carboxyl-terminal domain less antibody
    • Dynamin like protein antibody
    • Dynamin related protein 1 antibody
    • Dynamin-1-like protein antibody
    • Dynamin-like protein 4 antibody
    • Dynamin-like protein antibody
    • Dynamin-like protein IV antibody
    • Dynamin-related protein 1 antibody
    • DYNIV 11 antibody
    • EMPF antibody
    • EMPF1 antibody
    • FLJ41912 antibody
    • HdynIV antibody
    • VPS1 antibody
    see all

画像

  • Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
    Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
    All lanes : Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human cerebellum [PMID: 9422767]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling DRP1 with ab184248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human glioma [PMID: 25730670]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
    Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
    All lanes : Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 5 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 6 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 9 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-8: 3 minutes; Lane 9: 30 seconds.

  • Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
    Western blot - Anti-DRP1 antibody [EPR19275] (ab184248)
    All lanes : Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lane 2: 3 seconds; Lane 3: 10 seconds; Lane 4: 1 second; Lane 5: 5 seconds; Lane 6: 5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebellum [PMID: 9422767]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (ab184248)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebellum [PMID: 9422767]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-DRP1 antibody [EPR19275] (ab184248)
    Flow Cytometry (Intracellular) - Anti-DRP1 antibody [EPR19275] (ab184248)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DRP1 with ab184248 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-DRP1 antibody [EPR19275] (ab184248)
    Immunoprecipitation - Anti-DRP1 antibody [EPR19275] (ab184248)

    DRP1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184248 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184248 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10 μg (Input).

    Lane 2: ab184248 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184248 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-DRP1 antibody [EPR19275] (ab184248)
    Anti-DRP1 antibody [EPR19275] (ab184248)

参考文献 (8)

ab184248 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184248 は 8 報の論文で使用されています。

  • He J  et al. Effects of NBP on postoperative cognitive dysfunction in rats via Nrf 2/ARE pathway. Aging (Albany NY) 15:276-286 (2023). PubMed: 36626245
  • Xiao Z  et al. Tibial fracture surgery in elderly mice caused postoperative neurocognitive disorder via SOX2OT lncRNA in the hippocampus. Mol Brain 16:36 (2023). PubMed: 37098623
  • Zhang Z  et al. Chrono-Aerobic Exercise Optimizes Metabolic State in DB/DB Mice through CLOCK-Mitophagy-Apoptosis. Int J Mol Sci 23:N/A (2022). PubMed: 36012573
  • Portz P & Lee MK Changes in Drp1 Function and Mitochondrial Morphology Are Associated with the a-Synuclein Pathology in a Transgenic Mouse Model of Parkinson's Disease. Cells 10:N/A (2021). PubMed: 33924585
  • Yang J  et al. T-2 Toxin-Induced Oxidative Stress Leads to Imbalance of Mitochondrial Fission and Fusion to Activate Cellular Apoptosis in the Human Liver 7702 Cell Line. Toxins (Basel) 12:N/A (2020). PubMed: 31936883
  • Hou L  et al. ABT737 enhances ovarian cancer cells sensitivity to cisplatin through regulation of mitochondrial fission via Sirt3 activation. Life Sci 232:116561 (2019). PubMed: 31247208
  • Wang Y  et al. Necrostatin-1 ameliorates the pathogenesis of experimental autoimmune encephalomyelitis by suppressing apoptosis and necroptosis of oligodendrocyte precursor cells. Exp Ther Med 18:4113-4119 (2019). PubMed: 31611942
  • Cowan DB  et al. Transit and integration of extracellular mitochondria in human heart cells. Sci Rep 7:17450 (2017). PubMed: 29234096

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