Anti-Drebrin 抗体 (ab11068)
Key features and details
- Rabbit polyclonal to Drebrin
- Suitable for: ICC, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Drebrin antibody
Drebrin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Drebrin -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Chicken, Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to amino acids 22-42 of Human Drebrin.
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特記事項
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11068の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | (1) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. |
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| IHC-P | (4) |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 特記事項 |
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ICC
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa. ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively. -
組織特異性
Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. -
配列類似性
Contains 1 ADF-H domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 396496 Chicken
- Entrez Gene: 1627 Human
- Entrez Gene: 56320 Mouse
- Entrez Gene: 81653 Rat
- Omim: 126660 Human
- SwissProt: P18302 Chicken
- SwissProt: Q16643 Human
- SwissProt: Q9QXS6 Mouse
see all -
別名
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody (ab11068)This image is courtesy of Carl Hobbs, King's College London, United KingdomRabbit polyclonal to Drebrin (ab11068) used in IHC(Formalin/PFA-fixed paraffin-embedded sections; Standard stABCpx-DAB detection). ab11068 detecting Drebrin protein on Rat spinal cord E16 embryo sections fixed in formaldehyde. Antigen retrieval step: Heat mediated. 1% BSA as blocking agent for 10 mins at RT. Ab11068 was used at a dilution of 1/2500 incubated for 16 hours. Secondary Antibody is biotin labeled anti rabbit IgG (1/300). Drebrin positivity conformed to published localisation: essentially, many non-neuronal developing tissues were positive, eg: skeletal primary myotubes. This picture is of spinal cord floor plate region (x20). Nuclei were counterstained using Mayer's Haemalum.
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Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab11068)This image is a courtesy of Anonymous Abreviewab11068 staining Drebrin in mouse COR-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton x100 and blocking with 2% BSA was done at 250C for 15 minutes. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.25% gelatin in PBS) for 3 hours at 25°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab11068)ICC/IF image of ab11068 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab11068 は 25 報の論文で使用されています。
- Chen C et al. Neuronal paxillin and drebrin mediate BDNF-induced force transduction and growth cone turning in a soft-tissue-like environment. Cell Rep 40:111188 (2022). PubMed: 35977504
- Freitas RO et al. Nano-Infrared Imaging of Primary Neurons. Cells 10:N/A (2021). PubMed: 34685539
- Kushwaha R et al. Non-cell autonomous astrocyte-mediated neuronal toxicity in prion diseases. Acta Neuropathol Commun 9:22 (2021). PubMed: 33546775
- Xu MS et al. Cerebral Ischemia-Reperfusion Is Associated With Upregulation of Cofilin-1 in the Motor Cortex. Front Cell Dev Biol 9:634347 (2021). PubMed: 33777942
- Liu Y & Fan D The Preparation of Ginsenoside Rg5, Its Antitumor Activity against Breast Cancer Cells and Its Targeting of PI3K. Nutrients 12:N/A (2020). PubMed: 31963684