Anti-DRD5 抗体 (ab32620)
Key features and details
- Rabbit polyclonal to DRD5
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DRD5 antibody
DRD5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DRD5 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Chicken -
免疫原
Synthetic peptide within Human DRD5 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P21918 -
ポジティブ・コントロール
- Human brain tissue.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32620の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 5 µg/ml.
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特記事項 |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
組織特異性
Neuron-specific, localized primarily within limbic regions of the brain. -
関連疾患
Defects in DRD5 are a cause of benign essential blepharospasm (BEB) [MIM:606798]. BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1816 Human
- Omim: 126453 Human
- SwissProt: P21918 Human
- Unigene: 380681 Human
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別名
- D(1B) dopamine receptor antibody
- D(5) dopamine receptor antibody
- D1 beta dopamine receptor antibody
see all
画像
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Ab32620, at a 1/50 dilution, staining DRD5 in formalin fixed paraffin embedded human brain tissue by Immunohistochemistry.
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ICC/IF image of ab32620 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab32620 は 3 報の論文で使用されています。
- Xu W et al. Immunogenomic Characteristics of Cell-Death-Associated Genes with Prognostic Implications in Bladder Cancer. Front Immunol 13:909324 (2022). PubMed: 35898507
- Huo X et al. Methylation-based reclassification and risk stratification of skull-base chordomas. Front Oncol 12:960005 (2022). PubMed: 36439461
- Qian X et al. Dopamine Pathway Mediated by DRD5 Facilitates Tumor Growth via Enhancing Warburg Effect in Esophageal Cancer. Front Oncol 11:655861 (2021). PubMed: 33898321