Anti-DR4 抗体 [DR-4-02] - Low endotoxin, Azide free (ab14738)
Key features and details
- Mouse monoclonal [DR-4-02] to DR4 - Low endotoxin, Azide free
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free
DR4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [DR-4-02] to DR4 - Low endotoxin, Azide free -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human DR4. Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
DR-4-02 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14738の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 3 - 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use a concentration of 3 - 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. -
組織特異性
Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells. -
配列類似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8797 Human
- Omim: 603611 Human
- SwissProt: O00220 Human
- Unigene: 213467 Human
- Unigene: 591834 Human
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別名
- Apo2 antibody
- CD261 antibody
- Cytotoxic TRAIL receptor antibody
see all
画像
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Flow Cytometry analysis of DR4 expression on the surface of hematopoietic cell lines. Cells were stained with purified anti-DR4 antibodies (ab14738) followed by Goat anti-mouse IgG PE. Histograms: Red - Goat anti-mouse IgG PE (no primary antibody) Black - anti-DR4 comparative antibody Blue and Green - anti-DR4 (ab14738:DR-4-02) in duplicate staining
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Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (ab14738)Immunofluorescence staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with DR4 expression plasmid using anti-human DR4 (ab14738:DR-4-02).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab14738 は 2 報の論文で使用されています。
- Passante E et al. Systems analysis of apoptosis protein expression allows the case-specific prediction of cell death responsiveness of melanoma cells. Cell Death Differ 20:1521-31 (2013). PubMed: 23933815
- Franzen CA et al. Matrix protein CCN1 is critical for prostate carcinoma cell proliferation and TRAIL-induced apoptosis. Mol Cancer Res 7:1045-55 (2009). PubMed: 19584265