Anti-DPP9 抗体 [EPR26469-62] - BSA and Azide free (ab302904)

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Western blot - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR26469-62] to DPP9 - BSA and Azide free
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Unconjugated

製品名

Anti-DPP9 antibody [EPR26469-62] - BSA and Azide free
DPP9 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR26469-62] to DPP9 - BSA and Azide free
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-P, IPmore details
適用なし: Flow Cyt (Intra) or ICC/IF
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HeLa, HepG2, DU 145, 293T, NIH/3T3 and PC-12 whole cell lysate. Human liver and lung tissue lysate. Mouse liver tissue lysate. Rat liver and heart tissue lysate. IHC-P: Human cerebrum tissue. Human colon carcinoma tissue. Mouse and rat liver tissue. IP: DU 145 whole cell lysate. Mouse liver tissue lysate.
特記事項
AB302904 is a carrier free version of AB302903.

AB302903 does not react in: ICC/IF and intracellular flow cytometry with human samples.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.2
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR26469-62
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-DPP9 antibody [EPR26469-62] (ab302903)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab302904の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		Use at an assay dependent concentration. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		Use at an assay dependent concentration.

特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

追加情報

Is unsuitable for Flow Cyt (Intra) or ICC/IF.

機能

Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2.
組織特異性

Ubiquitously expressed, with highest levels in liver, heart and muscle, and lowest levels in brain.
配列類似性

Belongs to the peptidase S9B family. DPPIV subfamily.
細胞内局在

Cytoplasm > cytosol.
Target information above from: UniProt accession Q86TI2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 91039 Human
- Entrez Gene: 224897 Mouse
- Entrez Gene: 301130 Rat
- Omim: 608258 Human
- SwissProt: Q86TI2 Human
- SwissProt: Q8BVG4 Mouse
- Unigene: 515081 Human
- Unigene: 205535 Mouse
別名
- Dipeptidyl peptidase 9 antibody
- Dipeptidyl peptidase IV related protein 2 antibody
- Dipeptidyl peptidase IV-related protein 2 antibody
- Dipeptidyl peptidase IX antibody
- Dipeptidyl peptidase like protein 9 antibody
- Dipeptidyl peptidase-like protein 9 antibody
- Dipeptidylpeptidase 9 antibody
- Dipeptidylpeptidase IX antibody
- DKFZp762F117 antibody
- DP 9 antibody
- DP9 antibody
- DPLP 9 antibody
- DPLP9 antibody
- DPP 9 antibody
- DPP IX antibody
- DPP9 antibody
- DPP9_HUMAN antibody
- DPRP 2 antibody
- DPRP-2 antibody
- DPRP2 antibody
- FLJ16073 antibody
see all

Western blot - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

All lanes : Anti-DPP9 antibody [EPR26469-62] (ab302903) at 1/1000 dilution

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting DPP9, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 98 kDa
Observed band size: 98 kDa

Exposure time: 15 seconds

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

All lanes : Anti-DPP9 antibody [EPR26469-62] (ab302903) at 1/1000 dilution

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 98 kDa
Observed band size: 98 kDa

Exposure time: 26 seconds

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Western blot - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

All lanes : Anti-DPP9 antibody [EPR26469-62] (ab302903) at 1/1000 dilution

Lane 1 : Human liver tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 4 : DU145 (human prostate carcinoma epithelial cell), whole cell lysate
Lane 5 : Mouse liver tissue lysate
Lane 6 : Rat liver tissue lysate
Lane 7 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 98 kDa
Observed band size: 98 kDa

Exposure time: 26 seconds

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

Blocking/dilution buffer: 5% NFDM/TBST.

The higher MW band at approximately 300 kDa is likely to be DPP9 homodimer (PubMed: 16475979, PubMed: 29382749).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling DPP9 with ab302903 at 1/100 dilution (4.62 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human colon carcinoma is observed (PMID: 32734369). The section was incubated with ab302903 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling DPP9 with ab302903 at 1/100 dilution (4.62 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse liver is observed (PMID: 12459266). The section was incubated with ab302903 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling DPP9 with ab302903 at 1/100 dilution (4.62 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat liver is observed (PMID: 12459266). The section was incubated with ab302903 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling DPP9 with ab302903 at 1/100 dilution (4.62 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on human cerebrum at low expression level is observed. The section was incubated with ab302903 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

DPP9 was immunoprecipitated from 0.35 mg DU 145 (human prostate carcinoma epithelial cell), whole cell lysate with ab302903 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: DU 145 whole cell lysate 10 μg (Input)

Lane 2: ab302903 IP in DU 145 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302903 in DU 145 whole cell lysate

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 15 seconds.

Observed MW (kDa): 98.
Immunoprecipitation - Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

This data was developed using AB302903, the same antibody clone in a different buffer formulation.

DPP9 was immunoprecipitated from 0.35 mg mouse liver tissue lysate with ab302903 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: mouse liver tissue lysate 10 μg (Input)

Lane 2: ab302903 IP in mouse liver tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302903 in mouse liver tissue lysate

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 15 seconds.

Observed MW (kDa): 98.
Anti-DPP9 antibody [EPR26469-62] (BSA and Azide free) (AB302904)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

ab302904 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302904 は論文での使用が確認できていません。

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A