Anti-DPP9 抗体 - Catalytic domain (ab42080)
Key features and details
- Rabbit polyclonal to DPP9 - Catalytic domain
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DPP9 antibody - Catalytic domain
DPP9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DPP9 - Catalytic domain -
由来種
Rabbit -
特異性
This antibody recognises all three forms of DPP9. This antibody does not recognise other DPP family members. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Cow, Human -
免疫原
Synthetic peptide corresponding to Human DPP9.
(Peptide available asab44599) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab42080の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| WB | (3) |
1/1000 - 1/5000. Predicted molecular weight: 98 kDa. Recommended dilution 1/1000 with colourimetric substrates, 1/5000 dilution with chemiluminescent substrates. Dilution optimised using Chromogenic detection.
|
| IHC-P | (3) |
1/50.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 98 kDa. Recommended dilution 1/1000 with colourimetric substrates, 1/5000 dilution with chemiluminescent substrates. Dilution optimised using Chromogenic detection. |
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IHC-P
1/50. |
ターゲット情報
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機能
Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. -
組織特異性
Ubiquitously expressed, with highest levels in liver, heart and muscle, and lowest levels in brain. -
配列類似性
Belongs to the peptidase S9B family. DPPIV subfamily. -
細胞内局在
Cytoplasm > cytosol. - Information by UniProt
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参照データベース
- Entrez Gene: 521100 Cow
- Entrez Gene: 91039 Human
- Omim: 608258 Human
- SwissProt: Q86TI2 Human
- Unigene: 515081 Human
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別名
- Dipeptidyl peptidase 9 antibody
- Dipeptidyl peptidase IV related protein 2 antibody
- Dipeptidyl peptidase IV-related protein 2 antibody
see all
画像
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Western blot - Anti-DPP9 antibody - Catalytic domain (ab42080)All lanes : Anti-DPP9 antibody - Catalytic domain (ab42080) at 1/1000 dilution
Lane 1 : Cell media from human prostate cancer DU145 cells (treated with SF)
Lane 2 : Cell media from human prostate cancer DU145 cells (treated with RA)
Lane 3 : Cell media from human prostate cancer DU145 cells (treated with IL1-beta)
Predicted band size: 98 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody - Catalytic domain (ab42080)Ab42080 staining Human normal placenta. Staining is localised to the membrane and cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody - Catalytic domain (ab42080)This image is a courtesy of an Abreview submitted by Veronique Duboisab42080 staining DPP9 in bovine testicular tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Tris/EDTA buffer pH 9.0 and then the sample was blocked with 3% hydrogen peroxide in TBS for 10 minutes at 25°C. The sections were treated for 10 min with 3% H2O2 in TBS to block endogenous peroxidase activity and incubated subsequently for 30 min with 10% nonimmune goat serum to minimize nonspecific antibody binding. The primary antibody (1/50) was incubated with sample in TBS, 0.3% Triton X-100, 0.1 % BSA for 16 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-DPP9 antibody - Catalytic domain (ab42080)ICC/IF image of ab42080 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab42080 は 33 報の論文で使用されています。
- Clark KM et al. Chemical inhibition of DPP9 sensitizes the CARD8 inflammasome in HIV-1-infected cells. Nat Chem Biol 19:431-439 (2023). PubMed: 36357533
- Bettecken A et al. Dipeptidyl-Aminopeptidases 8 and 9 Regulate Autophagy and Tamoxifen Response in Breast Cancer Cells. Cells 12:N/A (2023). PubMed: 37626841
- Rao SD et al. M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome. Nat Chem Biol 18:565-574 (2022). PubMed: 35165443
- Bolgi O et al. Dipeptidyl peptidase 9 triggers BRCA2 degradation and promotes DNA damage repair. EMBO Rep 23:e54136 (2022). PubMed: 35912982
- Harapas CR et al. DPP9 deficiency: An inflammasomopathy that can be rescued by lowering NLRP1/IL-1 signaling. Sci Immunol 7:eabi4611 (2022). PubMed: 36112693