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AB133735

Anti-DPP3 抗体 [EPR9021(B)]

Anti-DPP3 antibody [EPR9021(B)]

5

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal DPP3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Dipeptidyl peptidase 3, Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, Dipeptidyl peptidase III, Enkephalinase B, DPP III, DPP3

2 Images
Western blot - Anti-DPP3 antibody [EPR9021(B)] (AB133735)
  • WB

Unknown

Western blot - Anti-DPP3 antibody [EPR9021(B)] (AB133735)

All lanes:

Western blot - Anti-DPP3 antibody [EPR9021(B)] (ab133735) at 1/10000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 83 kDa

false

OI-RD Scanning - Anti-DPP3 antibody [EPR9021(B)] (AB133735)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-DPP3 antibody [EPR9021(B)] (AB133735)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-DPP3 antibody [EPR9021(B)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR9021(B)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB97437

Anti-DPP3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DPP3 antibody (AB97437)

  • 4
  • 1
Primary Antibodies

AB217127

Anti-DPP3 antibody

Immunoprecipitation - Anti-DPP3 antibody (AB217127)

  • 0
  • 0
Primary Antibodies

AB133671

Anti-DPP3 antibody [EPR9020(B)]

RabMAb|Recombinant

Western blot - Anti-DPP3 antibody [EPR9020(B)] (AB133671)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB66440

Anti-GRIK2/GluK2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GRIK2/GluK2 antibody (AB66440)

  • 0
  • 0
Primary Antibodies

AB218242

Anti-ADAM9 antibody [EPR21924-164]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (AB218242)

  • 0
  • 0
Primary Antibodies

AB1187

HRP Anti-6X His tag® antibody

Western blot - HRP Anti-6X His tag® antibody (AB1187)

  • 4
  • 12
Primary Antibodies

AB274322

Anti-htrA1 antibody [EPR23240-64]

20ul selling size|KO Validated|RabMAb|Recombinant|Trial Size

Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (AB274322)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存温度
+4°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein DPP3 short for Dipeptidyl Peptidase 3 is an enzyme with a mass of approximately 82 kDa. It belongs to the M49 family of metallopeptidases and is widely expressed in human tissues including kidney heart liver and brain. This enzyme has the function of cleaving dipeptides from the N-terminus of polypeptides. Alternative names for DPP3 include DPP III and dipeptidyl peptidase III. By breaking down peptides DPP3 plays an important role in proteolysis.
Biological function summary

DPP3 influences several physiological processes. It is reported to participate in the degradation of enkephalins which are neuropeptides involved in regulating pain. DPP3 does not form part of a larger complex but operates independently within cells to execute its functions. By degrading its substrates DPP3 helps modulate peptide signaling pathways and maintain cellular homeostasis.

Pathways

DPP3 has significant involvement in the enkephalin degradation pathway which is essential in pain modulation and stress responses. It also plays a part in the ubiquitin-proteasome pathway facilitating the degradation of damaged or misfolded proteins. DPP3 interacts with proteins such as the ubiquitin-conjugating enzyme influencing protein turnover processes.

DPP3 links closely to cardiovascular diseases and cancer. Elevated levels of DPP3 are observed in heart failure where it may disrupt normal peptide balance and contribute to disease progression. Additionally its relationship with proteins such as angiotensin-converting enzyme (ACE) may affect blood pressure regulation. In certain cancers DPP3 expression changes can influence tumor growth by altering peptide-mediated signaling pathways with its interactions suggesting potential as a therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cleaves and degrades bioactive peptides, including angiotensin, Leu-enkephalin and Met-enkephalin (PubMed : 1515063, PubMed : 3233187). Also cleaves Arg-Arg-beta-naphthylamide (in vitro) (PubMed : 11209758, PubMed : 3233187, PubMed : 9425109).
See full target information DPP3
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 77:2881-2892 PubMed28416489

2017

NRF2 Induction Supporting Breast Cancer Cell Survival Is Enabled by Oxidative Stress-Induced DPP3-KEAP1 Interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Lu,Allen L Alcivar,Jianglin Ma,Tzeh Keong Foo,Susan Zywea,Amar Mahdi,Yanying Huo,Thomas W Kensler,Michael L Gatza,Bing Xia
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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