Anti-DP1 抗体 (ab186831)
Key features and details
- Rabbit polyclonal to DP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DP1 antibody
DP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human DP1 aa 1-290 (N terminal).
Database link: Q14186 -
ポジティブ･コントロール
- WB: LO2 and Raji cell lysates, mouse kidney tissue lysate. IHC: human lung and human colon damage tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186831の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/200.
|
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| WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 45 kDa.
|
| 特記事項 |
|---|
|
IHC-P
1/50 - 1/200. |
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WB
1/500 - 1/2000. Predicted molecular weight: 45 kDa. |
ターゲット情報
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機能
Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis. -
組織特異性
Highest levels in muscle. Also expressed in brain, placenta, liver and kidney. Lower levels in lung and pancreas. Not detected in heart. -
配列類似性
Belongs to the E2F/DP family. -
翻訳後修飾
Phosphorylation by E2F-1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7027 Human
- Entrez Gene: 21781 Mouse
- Omim: 189902 Human
- SwissProt: Q14186 Human
- SwissProt: Q08639 Mouse
- Unigene: 79353 Human
- Unigene: 925 Mouse
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別名
- DP 1 antibody
- DP1 antibody
- DRTF1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DP1 antibody (ab186831)
Paraffin-embedded human lung tissue stained for DP1 using ab186831 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-DP1 antibody (ab186831)All lanes : Anti-DP1 antibody (ab186831) at 1/3000 dilution
Lane 1 : LO2 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DP1 antibody (ab186831)Paraffin-embedded human colon damage stained for DP1 using ab186831 at 1/200 dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab186831 は 1 報の論文で使用されています。
- Zhou J et al. Identification of the E2F1-RAD51AP1 axis as a key factor in MGMT-methylated GBM TMZ resistance. Cancer Biol Med 20:385-400 (2023). PubMed: 37283490