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リコンビナント

Anti-Dopamine Receptor D1 抗体 [1-1-F11 s.E6] - BSA and Azide free (ab256556)

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Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1-1-F11 s.E6] to Dopamine Receptor D1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free
    Dopamine Receptor D1 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [1-1-F11 s.E6] to Dopamine Receptor D1 - BSA and Azide free

  • 由来種

    Rat

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: IHC-Fr

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    The details of the immunogen for this antibody are not available.

  • ポジティブ･コントロール

    • WB: Mouse and rat striatum lysates. IHC-P: Human rat and mouse corpus striatum, mouse brain FFPE tissue sections.

  • 特記事項

    ab256556 is a carrier free version of ab256555. 

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab256556の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 75-100 kDa (predicted molecular weight: 49 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 75-100 kDa (predicted molecular weight: 49 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for IHC-Fr.

ターゲット情報

  • 機能

    Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

  • 組織特異性

    Detected in caudate, nucleus accumbens and in the olfactory tubercle.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.

  • 細胞内局在

    Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
  • Information by UniProt

  • 参照データベース

    • 別名

      • D(1A) dopamine receptor antibody
      • D1A dopamine receptor antibody
      • DADR antibody
      • Dopamine D1 receptor antibody
      • dopamine receptor D1 antibody
      • DR D1 antibody
      • DR D1A antibody
      • DRD 1 antibody
      • DRD 1A antibody
      • DRD1 antibody
      • DRD1_HUMAN antibody
      • DRD1A antibody
      see all

    画像

    • Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
      Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
      All lanes : Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555) at 1/1000 dilution

      Lane 1 : Mouse striatum lysate
      Lane 2 : Rat striatum lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

      Predicted band size: 49 kDa
      Observed band size: 75-100 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      This data was developed using ab256555, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration:5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)

      This data was developed using ab256555, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)

      This data was developed using ab256555, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)

      This data was developed using ab256555, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)

      This data was developed using ab256555, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab256556 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab256556 は論文での使用が確認できていません。

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