Anti-Dopamine Receptor D1 抗体 [1-1-F11 s.E6] (ab256555)
![Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)](https://www.abcam.co.jp/ps/products/256/ab256555/Images/ab256555-488614-anti-dopamine-receptor-d1-antibody-11f11-se6-wb-mouse-rat-striatum.jpg "Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1-1-F11 s.E6] to Dopamine Receptor D1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6]
Dopamine Receptor D1 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [1-1-F11 s.E6] to Dopamine Receptor D1 -
由来種
Rat -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: IHC-Fr -
種交差性
交差種: Mouse, Rat -
免疫原
The details of the immunogen for this antibody are not available.
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ポジティブ･コントロール
- WB: Mouse and rat striatum lysates. IHC-P: Human, rat and mouse corpus striatum, mouse brain FFPE tissue sections.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
1-1-F11 s.E6 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab256555の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 75-100 kDa (predicted molecular weight: 49 kDa).
|
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| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 75-100 kDa (predicted molecular weight: 49 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
組織特異性
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
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参照データベース
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- SwissProt: Q61616 Mouse
- SwissProt: P18901 Rat
- Unigene: 54161 Mouse
- Unigene: 24039 Rat
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別名
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
画像
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Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)All lanes : Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555) at 1/1000 dilution
Lane 1 : Mouse striatum lysate
Lane 2 : Rat striatum lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 75-100 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking / Diluting buffer and concentration:5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)](https://www.abcam.co.jp/ps/products/256/ab256555/Images/ab256555-488610-anti-dopamine-receptor-d1-antibody-11f11-se6-ihcp-human-corpus-striatum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)](https://www.abcam.co.jp/ps/products/256/ab256555/Images/ab256555-488611-anti-dopamine-receptor-d1-antibody-11f11-se6-ihcp-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)](https://www.abcam.co.jp/ps/products/256/ab256555/Images/ab256555-488612-anti-dopamine-receptor-d1-antibody-11f11-se6-ihcp-mouse-corpus-striatum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)](https://www.abcam.co.jp/ps/products/256/ab256555/Images/ab256555-488613-anti-dopamine-receptor-d1-antibody-11f11-se6-ihcp-rat-corpus-striatum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab256555 は論文での使用が確認できていません。