Anti-Dopamine Receptor D1 抗体 [1-1-F11 s.E6]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555)

Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555)

Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555)

Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with anti dopamine receptor d1 antibody 11f11 se6 ihcp mouse cerebrumat 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555)

Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555)

Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• WB

Lab

Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555)

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1 - 2:

Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (<a href='/products/primary-antibodies/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556'>ab256556</a>)

Lane 1:

Mouse striatum lysate at 20 µg

Lane 2:

Rat striatum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 75-100 kDa

false