AB223130

Anti-Dopamine beta Hydroxylase 抗体 [EPR20385] - BSA and Azide free

Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Dopamine beta Hydroxylase antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, DBH

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on chromaffin cells of human adrenal gland medulla, and negative on adrenal cortex (PMID : 17535872, PMID : 17699566).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Immunohistochemical analysis of paraffin-embedded human adrenal pheochromocytoma tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on tumor cells of human adrenal pheochromocytoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Dopamine beta Hydroxylase was immunoprecipitated from 0.35 mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab209487 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab209487 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : SH-SY5Y whole cell lysate, 10 μg (Input).

Lane 2 : ab209487 IP in SH-SY5Y whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab209487 in SH-SY5Y whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

All lanes:

Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (<a href='/products/primary-antibodies/dopamine-beta-hydroxylase-antibody-epr20385-ab209487'>ab209487</a>)

Predicted band size: 69 kDa

Observed band size: 75 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on chromaffin cells of rat adrenal gland medulla, and negative on adrenal cortex (PMID : 17535872, PMID : 176995660).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on chromaffin cells of mouse adrenal gland medulla, and negative on adrenal cortex (PMID : 17535872, PMID : 176995660).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative controls : HeLa (PMID : 7688735; PMID : 9763470, PMID : 15585414, PMID : 10037744), C6 (PMID : 9763470, PMID : 7798950, PMID : 10037744), HepG2 (PMID : 7798950, PMID : 11943777, PMID : 15585414).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

All lanes:

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (<a href='/products/primary-antibodies/dopamine-beta-hydroxylase-antibody-epr20385-ab209487'>ab209487</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 75 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 minutes; Lane 2 : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

All lanes:

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (<a href='/products/primary-antibodies/dopamine-beta-hydroxylase-antibody-epr20385-ab209487'>ab209487</a>) at 1/1000 dilution

Lane 1:

Mouse adrenal gland lysate at 10 µg

Lane 2:

Rat adrenal gland lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 75 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20385

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Not recommended for use with fluorescent secondary antibodies in mouse IHC-P applications.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Not recommended for use with fluorescent secondary antibodies in mouse IHC-P applications; compatibility with rat and human species has not been tested.Tris/EDTA Buffer, pH9 (<a href='/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a>)." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Not recommended for use with fluorescent secondary antibodies in mouse IHC-P applications; compatibility with rat and human species has not been tested.Tris/EDTA Buffer, pH9 (<a href='/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a>)." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Not recommended for use with fluorescent secondary antibodies in mouse IHC-P applications; compatibility with rat and human species has not been tested.Tris/EDTA Buffer, pH9 (<a href='/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a>)." } } }

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製品の詳細

ab223130 is the carrier-free version of ab209487.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.

See full target information Dopamine beta-hydroxylase

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8882 PubMed41053097

2025

The Hippo terminal effector YAP boosts enterovirus replication in type 1 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Shirin Geravandi,Huan Liu,Heena Pahwa,Murali Krishna Madduri,Farah Atawneh,Adib Miraki Feriz,Sahar Rafizadeh,Annabelle Elisabeth Kruf,Mona Khazaei,Pouria Bahrami,David Gotti,Mohamed Elawour,Ruth M Elgamal,Ausilia Maria Grasso,David Bund,Blaz Lupse,Zahra Azizi,Omar Zabad,Karim Bouzakri,Marc Horwitz,Alberto Pugliese,Kathrin Maedler,Amin Ardestani

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dopamine beta Hydroxylase 抗体 [EPR20385] - BSA and Azide free

Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise