アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 03-4571-1344 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    製品タイプ別
    タンパク質・ペプチド
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    products/primary-antibodies/dok7-antibody-ab75049.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Adapters Transmembrane
Share by email

Anti-DOK7 抗体 (ab75049)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (3)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-DOK7 antibody (ab75049)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK7 antibody (ab75049)
  • Immunocytochemistry/ Immunofluorescence - Anti-DOK7 antibody (ab75049)

Key features and details

  • Rabbit polyclonal to DOK7
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
Product image
Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] - BSA and Azide free (ab271930)

関連製品

製品の概要

  • 製品名

    Anti-DOK7 antibody
  • 製品の詳細

    Rabbit polyclonal to DOK7
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from N-terminus of human DOK7.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Adapters
    • Transmembrane
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75049の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/50 - 1/100.
ICC/IF
1/500 - 1/1000.
特記事項
WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/50 - 1/100.
ICC/IF
1/500 - 1/1000.

ターゲット情報

  • 機能

    Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
  • 組織特異性

    Preferentially expressed in skeletal muscle and heart Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level).
  • 関連疾患

    Defects in DOK7 are the cause of familial limb-girdle myasthenia autosomal recessive (LGM) [MIM:254300]; also called congenital myasthenic syndrome type 1B or CMS1B. LGM is a congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
  • 配列類似性

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 細胞内局在

    Cell membrane. Cell junction > synapse. Accumulates at neuromuscular junctions.
  • Target information above from: UniProt accession Q18PE1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 285489 Human
    • Entrez Gene: 231134 Mouse
    • SwissProt: Q18PE1 Human
    • SwissProt: Q18PE0 Mouse
    • Unigene: 122110 Human
    • Unigene: 701584 Human
    • Unigene: 205483 Mouse
    • 別名

      • Docking protein 7 antibody
      • DOK 7 antibody
      • DOK7 antibody
      • DOK7_HUMAN antibody
      • Downstream of tyrosine kinase 7 antibody
      • Protein Dok-7 antibody
      see all

    画像

    • Western blot - Anti-DOK7 antibody (ab75049)
      Western blot - Anti-DOK7 antibody (ab75049)
      All lanes : Anti-DOK7 antibody (ab75049) at 1/500 dilution

      Lane 1 : extracts from mouse brain cells
      Lane 2 : extracts from mouse brain cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 53 kDa
      Observed band size: 53 kDa
      Additional bands at: 46 kDa, 80 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK7 antibody (ab75049)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK7 antibody (ab75049)
      Human brain tissue with ab75049 at 1/50 dilution, in the absence and presence of the immunising peptide.
    • Immunocytochemistry/ Immunofluorescence - Anti-DOK7 antibody (ab75049)
      Immunocytochemistry/ Immunofluorescence - Anti-DOK7 antibody (ab75049)
      HepG2 cells with ab75049 at 1/500 dilution, in the absence and presence of the immunising peptide.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (3)

    ab75049 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab75049 は 3 報の論文で使用されています。

    • Yue C  et al. DOK7 Inhibits Cell Proliferation, Migration, and Invasion of Breast Cancer via the PI3K/PTEN/AKT Pathway. J Oncol 2021:4035257 (2021). PubMed: 33552156
    • Huot JR  et al. Muscle weakness caused by cancer and chemotherapy is associated with loss of motor unit connectivity. Am J Cancer Res 11:2990-3001 (2021). PubMed: 34249440
    • Zhao H  et al. DOK7V1 influences the malignant phenotype of lung cancer cells through PI3K/AKT/mTOR and FAK/paxillin signaling pathways. Int J Oncol 54:381-389 (2019). PubMed: 30431081

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-DOK7 antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Tibialis anterior muscle)
    Specification
    Tibialis anterior muscle
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100, 3% in TBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Alejandro Gomez

    Verified customer

    投稿 Sep 10 2012

    Question

    Thanks again for offering us the chance to get a discount in our next purchase. Indeed our lab is interested in participating in your offer. So, we’ll purchase the antibody and try it on our frozen muscle samples from rat tissue, then we’ll send the results to you (I expect the procedure to take something between 1-2 months, considering that we might need to try several conditions and secondary antibodies, but hopefully not!).

    Therefore, I would like to ask if we can get a promotional code, so I can then proceed with the ordering of the antibody (ab75049).

    Many thanks for your help and looking forward to your reply.

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 05 2012

    Answer

    DISCOUNT CODE: 000000


    Expiration date: 05. October 2012

    I am very pleased to hear you would like to accept our offer and test ab75049 in IHC-Fr. This code will give you: the value of ab75049 a future order before the expiration date. To redeem this offer, please submit an Abreview forIHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jun 05 2012

    Question

    Product code: 75049

    Inquiry: Dear Sir or Madam, I’m A, PhD student at Maastricht University in The Netherlands (Department Neuroscience) and I’m currently working on a big project that involves studying the neuromuscular protein dok7. I’m particularly interested in optimizing a staining (immunofluorescence) for this protein in frozen slides of muscle tissue and I have tried that with two antibodies from **competitor name** Biotech., with poor results. I’ve noticed that you are offering an antibody for this protein (ab75049) that cross-reacts with mouse and seems to be suitable for IHC. I was wondering if perhaps your company is able to provide our lab with a small aliquote of the antibody in order to test whether it works in our samples or not, before we go and buy the antibody in bigger quantities. Do you think this is possible to arrange? Many thanks in advance for you help and looking forward to your reply. Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on May 31 2012

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.


    To our knowledge, ab75049 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab75049 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of ab75049 and can be used as value off a future order.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab75049 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab75049 so please wait for my reply before ordering.

    2. Purchase ab75049 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-Fr.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab75049 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on May 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2025 Abcam plc. All rights reserved.