Anti-DOK3 抗体 (ab1357)
Key features and details
- Goat polyclonal to DOK3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DOK3 antibody
DOK3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DOK3 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human DOK3 aa 450-550 (C terminal).
Database link: NP_079148.2 -
ポジティブ･コントロール
- Human PBMC lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1357の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function. -
組織特異性
Expressed in spleen. -
配列類似性
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
ドメイン
PTB domain mediates receptor interaction. -
翻訳後修飾
Constitutively tyrosine-phosphorylated.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase. -
細胞内局在
Cytoplasm. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 79930 Human
- Entrez Gene: 306760 Rat
- Omim: 611435 Human
- SwissProt: Q7L591 Human
- SwissProt: B2RYG7 Rat
- Unigene: 720849 Human
- Unigene: 19911 Rat
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別名
- Docking protein 3 antibody
- DOK 3 antibody
- DOK Like Protein antibody
see all
画像
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Western blot - Anti-DOK3 antibody (ab1357)Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg
Predicted band size: 57 kDaab1357 staining (1
Primary incubated for 1 hour. Detected by chemiluminescence.µ g/ml) of Human PBMC lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Western blot - Anti-DOK3 antibody (ab1357)All lanes : Anti-DOK3 antibody (ab1357) at 2 µg/ml
Lane 1 : Human Bone Marrow lysates
Lane 2 : Peripheral blood leukocyte lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 57 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab1357 は 3 報の論文で使用されています。
- Bounab Y et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . PubMed: 23820730
- Selimovic D et al. Apoptosis related protein-1 triggers melanoma cell death via interaction with the juxtamembrane region of p75 neurotrophin receptor. J Cell Mol Med 16:349-61 (2012). PubMed: 21418516
- Heckel T et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . PubMed: 19164586