AB197587

Anti-DOHH 抗体 [EPR16730]

Anti-DOHH antibody [EPR16730]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal DOHH antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

HLRC1, DOHH, Deoxyhypusine hydroxylase, hDOHH, Deoxyhypusine dioxygenase, Deoxyhypusine monooxygenase, HEAT-like repeat-containing protein 1

2 Images

Supplier Data

Immunoprecipitation - Anti-DOHH antibody [EPR16730] (AB197587)

DOHH was immunoprecipitated from SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell extract with ab197587 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197587 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : SH-SY5Y whole cell extract 10 μg (Input). Lane 2 : ab197587 IP in SH-SY5Y whole cell extract. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab197587 in SH-SY5Y whole cell extract

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DOHH antibody [EPR16730] (ab197587)

Predicted band size: 33 kDa

Observed band size: 33 kDa

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Supplier Data

Western blot - Anti-DOHH antibody [EPR16730] (AB197587)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DOHH antibody [EPR16730] (ab197587) at 1/5000 dilution

Lane 1:

LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

Lane 2:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Exposure time: 1min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16730

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The deoxyhypusine hydroxylase (DOHH) enzyme also known as hDOHH catalyzes the final step in the post-translational synthesis of the unusual amino acid hypusine. DOHH modifies deoxyhypusine into hypusine by hydroxylation a reaction critical in activating the eukaryotic initiation factor 5A (eIF5A). The human DOHH protein has a mass of approximately 36 kDa and is ubiquitously expressed in various tissues reflecting its general significance in protein processing.

Biological function summary

Proteins such as DOHH play important roles in cellular growth and proliferation. DOHH functions by converting inactive eIF5A precursors into their active form which can influence mRNA translation. This enzyme does not form part of a larger complex with other proteins but its activity is essential for the hypusine modification necessary to activate eIF5A directly influencing protein synthesis.

Pathways

DOHH operates in the hypusine biosynthesis pathway which is important for cell proliferation and differentiation. DOHH acts after the enzyme deoxyhypusine synthase (DHS) introduces a deoxyhypusine residue into eIF5A illustrating the close relationship between these two proteins in this cascade. The activation of eIF5A through hypusination impacts diverse pathways that regulate cell cycle progression and apoptosis.

DOHH activity has implications in cancer and neurodegenerative diseases. Altered expression or malfunction of DOHH can influence eIF5A activity affecting cell growth and survival and therefore contributing to oncogenesis. In neurodegenerative conditions abnormal hypusine synthesis may disrupt neuronal integrity and function. The linked protein eIF5A plays a role in these diseases demonstrating the broader impact of DOHH on health outcomes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine (PubMed : 16371467, PubMed : 16533814, PubMed : 19706422). Hypusination is unique to mature eIF-5A factor and is essential for its function (By similarity).

See full target information DOHH

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 56:1633-1643 PubMed39308302

2024

knockdown induces cell cycle arrest and apoptosis in breast cancer cells through the ZBTB16/ANXA7/Cyclin B1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Di Liu,Qin Du,Yuxuan Zhu,Yize Guo,Ya Guo

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOHH 抗体 [EPR16730]

Anti-DOHH antibody [EPR16730]

Immunoprecipitation - Anti-DOHH antibody [EPR16730] (AB197587)

Western blot - Anti-DOHH antibody [EPR16730] (AB197587)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise