Anti-DOCK9/Trg 抗体 (ab70272)
Key features and details
- Rabbit polyclonal to DOCK9/Trg
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DOCK9/Trg antibody
DOCK9/Trg 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DOCK9/Trg -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Opossum, Orangutan
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免疫原
Synthetic peptide, corresponding to amino acids 1-50 of Human DOCK9 (NP_056111.1)
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ポジティブ･コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70272の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/15000. Detects a band of approximately 236 kDa (predicted molecular weight: 236 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/5000 - 1/15000. Detects a band of approximately 236 kDa (predicted molecular weight: 236 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation. -
組織特異性
Widely expressed, with highest expression in heart and placenta. Expressed at intermediate level in kidney, brain, lung and skeletal muscle. -
配列類似性
Belongs to the DOCK family.
Contains 1 DHR-1 domain.
Contains 1 DHR-2 domain.
Contains 1 PH domain. -
ドメイン
The DHR-2 domain is necessary and sufficient for the GEF activity. -
細胞内局在
Endomembrane system. Associated with membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 23348 Human
- Omim: 607325 Human
- SwissProt: Q9BZ29 Human
- Unigene: 596105 Human
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別名
- Cdc42 guanine nucleotide exchange factor zizimin 1 antibody
- Cdc42 guanine nucleotide exchange factor zizimin-1 antibody
- Dedicator of cytokinesis protein 9 antibody
see all
画像
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Immunoprecipitation - Anti-DOCK9/Trg antibody (ab70272)
Immunoprecipitation of HeLa using 3ug/mL ab70272
Lane 1: ab70272
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Western blot - Anti-DOCK9/Trg antibody (ab70272)All lanes : Anti-DOCK9/Trg antibody (ab70272)
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 236 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab70272 は 1 報の論文で使用されています。
- Wieczfinska J et al. The whole-genome expression analysis of peripheral blood mononuclear cells from aspirin sensitive asthmatics versus aspirin tolerant patients and healthy donors after in vitro aspirin challenge. Respir Res 16:147 (2015). WB . PubMed: 26646719