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リコンビナントRabMAb

Anti-DOCK8 抗体 [EPR12511] (ab175208)

Submit a review Submit a question References (4)
Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)
  • Western blot - Anti-DOCK8 antibody [EPR12511] (ab175208)
  • Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)
  • Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] (ab175208)
  • Anti-DOCK8 antibody [EPR12511] (ab175208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12511] to DOCK8
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-DOCK8 antibody [EPR12511]
    DOCK8 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12511] to DOCK8

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human DOCK8. The exact sequence is proprietary.
    Database link: Q8NF50

  • ポジティブ･コントロール

    • Raji and THP1 cell lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12511

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab175208の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 239 kDa.
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 239 kDa.
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP.

  • 関連疾患

    Defects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES) [MIM:243700]. It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement.

  • 配列類似性

    Belongs to the DOCK family.
    Contains 1 DHR-1 (CZH-1) domain.
    Contains 1 DHR-2 (CZH-2) domain.

  • ドメイン

    The DHR-2 domain may mediate some GEF activity.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 1200017A24Rik antibody
      • Dedicator of cytokinesis protein 8 antibody
      • DOCK8 antibody
      • DOCK8_HUMAN antibody
      • Epididymis luminal protein 205 antibody
      • HEL 205 antibody
      • MRD2 antibody
      • ZIR8 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)
      Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)

      Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Western blot - Anti-DOCK8 antibody [EPR12511] (ab175208)
      Western blot - Anti-DOCK8 antibody [EPR12511] (ab175208)
      All lanes : Anti-DOCK8 antibody [EPR12511] (ab175208) at 1/10000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 239 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)
      Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)

      Immunofluorescent staining of Raji cells labeling DOCK8 with ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).

    • Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] (ab175208)
      Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] (ab175208)

      Intracellular flow cytometric analysis of permeabilized THP1 cells labeling DOCK8with ab175208at 1/10 dilution (red) compared to a Rabbit IgG (green). 

       

       

    • Anti-DOCK8 antibody [EPR12511] (ab175208)
      Anti-DOCK8 antibody [EPR12511] (ab175208)

    参考文献 (4)

    ab175208 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab175208 は 4 報の論文で使用されています。

    • Meshaal SS  et al. Diagnosis of DOCK8 deficiency using Flow cytometry Biomarkers: an Egyptian Center experience. Clin Immunol 195:36-44 (2018). PubMed: 30048691
    • Cao M  et al. Mechanisms of Impaired Neutrophil Migration by MicroRNAs in Myelodysplastic Syndromes. J Immunol 198:1887-1899 (2017). PubMed: 28130497
    • Qin T  et al. Novel DOCK8 gene mutations lead to absence of protein expression in patients with hyper-IgE syndrome. Immunol Res N/A:N/A (2015). PubMed: 26659092
    • Kruithof-de Julio M  et al. Canonical Wnt signaling regulates Nkx3.1 expression and luminal epithelial differentiation during prostate organogenesis. Dev Dyn 242:1160-71 (2013). PubMed: 23813564

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