Anti-DOCK4 抗体 (ab85723)
Key features and details
- Rabbit polyclonal to DOCK4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DOCK4 antibody -
製品の詳細
Rabbit polyclonal to DOCK4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide, corresponding to a region within amino acids 1725-1775 of Human DOCK4 NP_055520.3
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035), 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85723の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 225 kDa.
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| IP |
Use at 3-5 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 225 kDa. |
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IP
Use at 3-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in regulation of adherens junction between cells. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP. -
組織特異性
Widely expressed at low level. Highly expressed in skeletal muscle, prostate and ovary. -
配列類似性
Belongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
Contains 1 SH3 domain. -
ドメイン
The DHR-2 domain probably mediates the GEF activity. -
細胞内局在
Endomembrane system. - Information by UniProt
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参照データベース
- Entrez Gene: 9732 Human
- Entrez Gene: 238130 Mouse
- Omim: 607679 Human
- SwissProt: Q8N1I0 Human
- SwissProt: P59764 Mouse
- Unigene: 654652 Human
- Unigene: 341423 Mouse
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別名
- Dedicator of cytokinesis protein 4 antibody
- Dock4 antibody
- DOCK4_HUMAN antibody
- KIAA0716 antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK4 antibody (ab85723)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DOCK4 with ab85723 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-DOCK4 antibody (ab85723)All lanes : Anti-DOCK4 antibody (ab85723) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 225 kDa
Observed band size: 225 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-DOCK4 antibody (ab85723)Detection of Human DOCK4 by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded)using ab85723 at 3ug/mg lysate. Subsequent Western blot detection was performed using 0.4 ug/ml ab85723. Detection: Chemiluminescence with exposure times of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab85723 は 4 報の論文で使用されています。
- Li H et al. Long Noncoding RNA EBLN3P Promotes the Progression of Liver Cancer via Alteration of microRNA-144-3p/DOCK4 Signal. Cancer Manag Res 12:9339-9349 (2020). PubMed: 33061623
- Guo D et al. Autism-like social deficit generated by Dock4 deficiency is rescued by restoration of Rac1 activity and NMDA receptor function. Mol Psychiatry N/A:N/A (2019). PubMed: 31388105
- Huang L et al. SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis. Nature 569:565-569 (2019). PubMed: 31019307
- Frank SR et al. p190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity. J Cell Biol 217:3183-3201 (2018). PubMed: 29934311