Anti-DOCK10 抗体 (ab75258)
Key features and details
- Rabbit polyclonal to DOCK10
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DOCK10 antibody -
製品の詳細
Rabbit polyclonal to DOCK10 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
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免疫原
Synthetic peptide within Human DOCK10 aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_055504.2
Database link: Q96BY6 -
ポジティブ･コントロール
- HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75258の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 250 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 250 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
DOCK10 is a potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Two named isoforms exist, produced by alternative splicing. -
参照データベース
- Entrez Gene: 55619 Human
- Omim: 611518 Human
- SwissProt: Q96BY6 Human
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別名
- 9330153B10Rik antibody
- A630054M16Rik antibody
- dedicator of cytokinesis 10 antibody
see all
画像
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Western blot - Anti-DOCK10 antibody (ab75258)All lanes : Anti-DOCK10 antibody (ab75258) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 250 kDa
Observed band size: 250 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-DOCK10 antibody (ab75258)Detection of DOCK10 by Western Blot of Immunprecipitate.
ab75258 at 1µg/ml staining DOCK10 in HeLa whole cell lysate immunoprecipitated using ab75258 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Detection: chemiluminescence with exposure time of 1 minute.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab75258 は論文での使用が確認できていません。