Anti-Dnmt3L 抗体 (ab3493)
Key features and details
- Rabbit polyclonal to Dnmt3L
- Suitable for: IP, IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Dnmt3L antibody
Dnmt3L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dnmt3L -
由来種
Rabbit -
特異性
This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. -
アプリケーション
適用あり: IP, IHC-P, ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Dnmt3L aa 152-164 conjugated to keyhole limpet haemocyanin.
Sequence:C-GLLQRRRKWRSQL
Database link: Q9UJW3 -
特記事項
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
一次抗体 備考
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3493の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP | (6) |
1/100.
|
IHC-P |
1/500.
|
|
ELISA |
1/1000 - 1/3000.
|
|
WB | (8) |
1/500. Predicted molecular weight: 44 kDa.
|
特記事項 |
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IP
1/100. |
IHC-P
1/500. |
ELISA
1/1000 - 1/3000. |
WB
1/500. Predicted molecular weight: 44 kDa. |
ターゲット情報
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機能
Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. -
組織特異性
Expressed at low levels in several tissues including testis, ovary, and thymus. -
配列類似性
Belongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 29947 Human
- Omim: 606588 Human
- SwissProt: Q9UJW3 Human
- Unigene: 592165 Human
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別名
- Cytosine 5 methyltransferase 3 like protein antibody
- DNA (cytosine 5 ) methyltransferase 3 like antibody
- DNA (cytosine-5)-methyltransferase 3-like antibody
see all
画像
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Transfection of U2OS cells (10cm dish) using 3
µ g of GAL4-DNMT3L. Protein extraction using IPH 150mM. IP and WB conditions, see Fuks et al. (2000). Nature Genetics 24: 88-91.WB using 0.4µ g/ml in TBS milk 2%, BSA 0.5%. Dectection using ECL.Note: ab3493 worked in WB following IP, but did not work in a straight WB.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab3493 は 15 報の論文で使用されています。
- Uysal F et al. DNMT enzymes differentially alter global DNA methylation in a stage-dependent manner during spermatogenesis. Andrologia 54:e14357 (2022). PubMed: 34997784
- Wang Y et al. Retinoic acid induces NELFA-mediated 2C-like state of mouse embryonic stem cells associates with epigenetic modifications and metabolic processes in chemically defined media. Cell Prolif 54:e13049 (2021). PubMed: 33960560
- Wu B et al. Activin A and BMP4 Signaling Expands Potency of Mouse Embryonic Stem Cells in Serum-Free Media. Stem Cell Reports 14:241-255 (2020). PubMed: 32032551
- Ishihara T et al. DNA methylation dynamics in the germline of the marsupial tammar wallaby, Macropus eugenii. DNA Res 26:85-94 (2019). PubMed: 30535324
- Peng J et al. The detrimental effects of glucocorticoids exposure during pregnancy on offspring's cardiac functions mediated by hypermethylation of bone morphogenetic protein-4. Cell Death Dis 9:834 (2018). PubMed: 30082698