Anti-Dnmt3a 抗体 - N-terminal

• IP

Supplier Data

Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (AB227725)

Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract with ab227725. Western blot was performed from the immunoprecipitate using ab227725 at 1/2000 dilution. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : HEK-293T nuclear extract 40 μg (Input).

Lane 2 : 4 μg preimmune rabbit IgG instead of ab227725 in HEK-293T nuclear extract.

Lane 3 : ab227725 IP in HEK-293T nuclear extract.

All lanes:

Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab227725)

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB227725)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract at 30 µg

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB227725)

Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with ab227725. Western blot was performed from the immunoprecipitate using ab227725. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : HEK-293T whole cell exract (Input).

Lane 2 : Control IgG instead of ab227725 in HEK-293T whole cell extract.

Lane 3 : ab227725 IP in HEK-293T whole cell extract.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725)

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB227725)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/3000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lanes 2 - 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Dnmt3a shRNA, whole cell extract at 30 µg

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB227725)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/1000 dilution

All lanes:

Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB227725)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 102 kDa

false