Anti-Dnmt3a 抗体 (ab16704)
Key features and details
- Rabbit polyclonal to Dnmt3a
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Dnmt3a antibody
Dnmt3a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dnmt3a -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to amino acids 10-118 of Human Dnmt3a.
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ポジティブ･コントロール
- ICC: Ntera-2 whole cell lysate. IHC-P: Human testis tissue
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特記事項
It is strongly suggested that Sodium Azide is added to this stock upon thawing and storage at 4 degrees C.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16704の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200.
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| ICC |
1/500.
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| 特記事項 |
|---|
|
IHC-P
1/200. |
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ICC
1/500. |
ターゲット情報
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機能
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
組織特異性
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
配列類似性
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
ドメイン
The PWWP domain is essential for targeting to pericentric heterochromatin. -
翻訳後修飾
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
細胞内局在
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 1788 Human
- Omim: 602769 Human
- SwissProt: Q9Y6K1 Human
- Unigene: 515840 Human
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別名
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
画像
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Immunocytochemistry - Anti-Dnmt3a antibody (ab16704)Immunocytochemistry analysis of Ntera-2 cells labeling Dnmt3a with ab16704 followed by Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody (ab16704)Immunohistochemistry analysis of human testis labeling Dnmt3a with ab16704 at 1/200. Hematoxylin was used as counterstain.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab16704 は 7 報の論文で使用されています。
- Szigeti KA et al. Global DNA hypomethylation of colorectal tumours detected in tissue and liquid biopsies may be related to decreased methyl-donor content. BMC Cancer 22:605 (2022). PubMed: 35655145
- Huang YH et al. Systematic Profiling of DNMT3A Variants Reveals Protein Instability Mediated by the DCAF8 E3 Ubiquitin Ligase Adaptor. Cancer Discov N/A:N/A (2021). PubMed: 34429321
- Abdalla BA et al. A Novel Dnmt3a1 Transcript Inhibits Adipogenesis. Front Physiol 9:1270 (2018). PubMed: 30333755
- Yossifoff M et al. Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter. Eur J Neurosci 28:2267-77 (2008). WB, EMSA ; Chicken . PubMed: 19046370
- Loriot A et al. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem 281:10118-26 (2006). PubMed: 16497664
- Robert MF et al. DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat Genet 33:61-5 (2003). PubMed: 12496760
- Beaulieu N et al. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. J Biol Chem 277:28176-81 (2002). PubMed: 12015329
