Anti-DNA/RNA Damage 抗体 [15A3] (ab62623)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)](https://www.abcam.co.jp/ps/products/62/ab62623/Images/ab62623-308668-anti-dna-rna-damage-antibody-15a3-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)")
Key features and details
- Mouse monoclonal [15A3] to DNA/RNA Damage
- Suitable for: IHC-P, ICC, ELISA, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-DNA/RNA Damage antibody [15A3]
DNA/RNA Damage 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [15A3] to DNA/RNA Damage -
由来種
Mouse -
特異性
Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
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アプリケーション
適用あり: IHC-P, ICC, ELISA, IHC-Frmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to DNA/RNA Damage. 8-hydroxy-guanosine-BSA and -casein conjugates.
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ポジティブ･コントロール
- IHC-P: Mouse inflammed colon and backskin tissues. Fresh IHC: Ischemic rat brain tissue.
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特記事項
Please see the protocol booklet link below for recommended IHC and ICC staining procedure
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
15A3 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62623の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (4) |
Use a concentration of 1 - 10 µg/ml.
Dilute antibody in PBS containing 0.3% Triton X-100, 0.08% sodium azide and 2% normal goat serum. |
| ICC | (1) |
Use at an assay dependent concentration.
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| ELISA | (2) |
Use at an assay dependent concentration.
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| AP |
Use at an assay dependent concentration.
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| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| 特記事項 |
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IHC-P
Use a concentration of 1 - 10 µg/ml. Dilute antibody in PBS containing 0.3% Triton X-100, 0.08% sodium azide and 2% normal goat serum. |
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ICC
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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AP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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関連性
In intact animals, lesions (adducts) excised from DNA are transported from the cell through the circulation and excreted in urine. In bacteria, DNA adducts are excreted directly into the medium. In either case, the adducts can be assayed as a measure of oxidative damage to DNA. In particular, Oxo-8-dG (8-Oxo-7,8-dihydro-2'-deoxyguanosine) serves as an excellent marker for DNA damage produced by oxidants because it represents one of the major products generated by a wide array of treatments associated with oxidant damage such as that produced by irradiation and various carcinogens and because it is implicated in spontaneous transversion mutagenesis. Oxo-8-Gua (8-oxo-7,8-dihydroguanine) is one of the most common DNA lesions resulting from reactive oxygen species and can result in a mismatched pairing with adenine resulting in G to T and C to A substitutions in the genome. In humans, it is primarily repaired by DNA glycosylase OGG1. It can be caused by ionizing radiation, in connection with oxidative metabolism. Oxo-8-G (8-oxo-7,8-dihydroguanosine) is classified as an oxidized ribonucleotide, and is primarily used in studies of oxidative RNA damage and associated RNA repair and RNA turnover mechanisms within the cell. In the cell, Oxo-8-G RNA lesions are formed by reaction with reactive oxygen species (ROS) generated either via normal oxidative metabolic processes, UV ionizing radiation, or exposure to oxidative agents. Oxidative RNA damage can lead to defects in protein synthesis, for example, decreased rates of protein synthesis and production of aggregated or truncated peptides, with important implications in aging and neurodegenerative disorders and artherosclerosis. -
別名
- 7,8-Dihydro-8-oxo-2'-deoxyguanosine antibody
- 7,8-Dihydro-8-oxodeoxyguanosine antibody
- 7,8-Dihydro-8-oxoguanine antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)
Bouin’s Fixative, paraffin-embedded mouse backskin tissue stained for DNA/RNA Damage using ab62623 (1 hr at RT) at 1/100 dilution in immunohistochemical analysis.
Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 for 1 hour at RT.
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![Immunocytochemistry - Anti-DNA/RNA Damage antibody [15A3] (ab62623)](https://www.abcam.co.jp/ps/products/62/ab62623/Images/ab62623-308669-ab6262340675.jpg)
Immunocytochemistry - Anti-DNA/RNA Damage antibody [15A3] (ab62623)This image is courtesy of an anonymous Abreview.Mouse hepatocytes stained for DNA/RNA Damage (green) using ab62623 at 1/500 dilution in ICC/IF, followed by Alexa-Fluor®488 conjugated Goat Anti-Mouse IgG (H+L).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)](https://www.abcam.co.jp/ps/products/62/ab62623/Images/8-Hydroxyguanosine-Primary-antibodies-ab62623-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)Left panel: ab62623 (1/1000 for 16 hours at RT) staining in ischemic rat brain tissue (fresh samples).
Center panel: DAPI staining
Right panel: mergedSecondary Antibody: Alexa Fluor®546 Goat Anti-mouse (Red) at 1/500 for 1 hour at RT.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)](https://www.abcam.co.jp/ps/products/62/ab62623/Images/ab62623-254256-anti-dna-rna-damage-antibody-15a3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)Image is courtesy of an AbReview submitted by Mr Helder Fonseca.Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labeling with ab62623 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A secondary was not used, but ab93695 detection kit was used for signal amplification.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)](https://www.abcam.co.jp/ps/products/62/ab62623/Images/8-Hydroxyguanosine-Primary-antibodies-ab62623-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)This image is a courtesy of Anonymous Abreviewab62623 staining in rat liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K solution and then blocking for 20 minutes at 37°C was performed. The primary antibody was diluted 1/4000 and incubated with sample for 2 hours at 37°C. A Biotin conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/200 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)](https://www.abcam.co.jp/ps/products/62/ab62623/Images/ab62623-308667-ab626231.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA/RNA Damage antibody [15A3] (ab62623)Paraffin-embedded mouse inflammed colon tissue stained for DNA/RNA Damage using ab62623 at 1/1,000,000 dilution (12 hrs at 4°C) in immunohistochemical analysis.
Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (167)
ab62623 は 167 報の論文で使用されています。
- Liao W et al. Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function. Br J Pharmacol 180:1056-1071 (2023). PubMed: 36440573
- Wang Q et al. Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription. J Bone Miner Res 38:427-442 (2023). PubMed: 36625422
- Owembabazi E et al. Co-administration of alcohol and combination antiretroviral therapy (cART) in male Sprague Dawley rats: A study on testicular morphology, oxidative and cytokines perturbations. Anat Cell Biol 56:236-251 (2023). PubMed: 36759974
- Yang Y et al. Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage. Front Immunol 14:1089576 (2023). PubMed: 36761756
- Zhu TT et al. Melanin-like polydopamine nanoparticles mediating anti-inflammatory and rescuing synaptic loss for inflammatory depression therapy. J Nanobiotechnology 21:52 (2023). PubMed: 36765377