Anti-DnaK 抗体 [8E2/2] (ab69617)
Key features and details
- Mouse monoclonal [8E2/2] to DnaK
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG1
製品の概要
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製品名
Anti-DnaK antibody [8E2/2]
DnaK 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8E2/2] to DnaK -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Escherichia coli -
免疫原
Full length protein corresponding to Escherichia coli DnaK.
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特記事項
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
8E2/2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69617の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
ターゲット情報
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関連性
DnaK is the prokaryotic analogue of eukaryotic Hsp70. Heat shock proteins applies to a group of proteins that assist in the assembly, folding, and translocation of other proteins. In addition, they protect the cell against heat injury or other forms of stress. All cells, prokaryotic and eukaryotic, are able to respond to different cellular stresses by synthesizing these proteins. Heat shock proteins are highly conserved, ubiquitously distributed, and involved in important aspects of viral and bacterial infections, autoimmune diseases, and in cancer immunity. Two families of molecular chaperones have been identified. The members of the Hsp70 family (DnaK/DnaJ/GrpE) bind to the growing polypeptide chain and prevent its premature folding. The chaperonin family (GroEL and GroES) assists in correct folding when the complete polypeptide chain is formed and is transported into the cytosol or mitochondria. All the major heat shock proteins help to suppress irreversible unfolding reactions. These protein folding 'assistants' may have important functions in amyloid diseases where incorrectly folded proteins accumulate as folded aggregates. -
細胞内局在
Cytoplasm. Cell inner membrane; Peripheral membrane protein. -
参照データベース
- Entrez Gene: 944750 Escherichia coli
- SwissProt: P0A6Y8 Escherichia coli
- SwissProt: Q1RGI8 Escherichia coli
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別名
- Chaperone Hsp70 antibody
- Chaperone protein dnaK antibody
- Co chaperone with DnaJ antibody
see all
画像
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All lanes :
Lane 1 : DnaK active recombinant protein
Lane 2 : E.coli cell lysate.
Predicted band size: 70 kDaThis image was generated using the ascites version of the product.
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Anti-DnaK antibody [8E2/2] (ab69617) at 0.1 µg/ml + E.Coli DnaK protein
Predicted band size: 70 kDa
Observed band size: 70 kDaThis image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (37)
ab69617 は 37 報の論文で使用されています。
- Liu B et al. Enterohaemorrhagic E. coli utilizes host- and microbiota-derived L-malate as a signaling molecule for intestinal colonization. Nat Commun 14:7227 (2023). PubMed: 37945607
- Branton WG et al. Microbial molecule ingress promotes neuroinflammation and brain CCR5 expression in persons with HIV-associated neurocognitive disorders. Brain Behav Immun 107:110-123 (2023). PubMed: 36202168
- Frumkin I & Laub MT Selection of a de novo gene that can promote survival of Escherichia coli by modulating protein homeostasis pathways. Nat Ecol Evol 7:2067-2079 (2023). PubMed: 37945946
- Liu J et al. Mechanistic insights into the subversion of the linear ubiquitin chain assembly complex by the E3 ligase IpaH1.4 of Shigella flexneri. Proc Natl Acad Sci U S A 119:e2116776119 (2022). PubMed: 35294289
- Cohen H et al. Intracellular Salmonella Paratyphi A is motile and differs in the expression of flagella-chemotaxis, SPI-1 and carbon utilization pathways in comparison to intracellular S. Typhimurium. PLoS Pathog 18:e1010425 (2022). PubMed: 35381053