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  1. Link

    products/primary-antibodies/dnak-antibody-8e22-ab69617.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-DnaK 抗体 [8E2/2] (ab69617)

  • Datasheet
  • SDS
Reviews (2)Q&A (6)References (37)

Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
  • Western blot - Anti-DnaK antibody [8E2/2] (ab69617)

Key features and details

  • Mouse monoclonal [8E2/2] to DnaK
  • Suitable for: WB
  • Reacts with: Escherichia coli
  • Isotype: IgG1

こちらの製品もご検討ください

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二次抗体
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-DnaK antibody [8E2/2]
    DnaK 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [8E2/2] to DnaK
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Escherichia coli
  • 免疫原

    Full length protein corresponding to Escherichia coli DnaK.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • 特記事項(精製)

    Purified from TCS.
  • ポリ/モノ

    モノクローナル
  • クローン名

    8E2/2
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant DnaK protein (ab51121)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab69617の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

ターゲット情報

  • 関連性

    DnaK is the prokaryotic analogue of eukaryotic Hsp70. Heat shock proteins applies to a group of proteins that assist in the assembly, folding, and translocation of other proteins. In addition, they protect the cell against heat injury or other forms of stress. All cells, prokaryotic and eukaryotic, are able to respond to different cellular stresses by synthesizing these proteins. Heat shock proteins are highly conserved, ubiquitously distributed, and involved in important aspects of viral and bacterial infections, autoimmune diseases, and in cancer immunity. Two families of molecular chaperones have been identified. The members of the Hsp70 family (DnaK/DnaJ/GrpE) bind to the growing polypeptide chain and prevent its premature folding. The chaperonin family (GroEL and GroES) assists in correct folding when the complete polypeptide chain is formed and is transported into the cytosol or mitochondria. All the major heat shock proteins help to suppress irreversible unfolding reactions. These protein folding 'assistants' may have important functions in amyloid diseases where incorrectly folded proteins accumulate as folded aggregates.
  • 細胞内局在

    Cytoplasm. Cell inner membrane; Peripheral membrane protein.
  • 参照データベース

    • Entrez Gene: 944750 Escherichia coli
    • SwissProt: P0A6Y8 Escherichia coli
    • SwissProt: Q1RGI8 Escherichia coli
    • 別名

      • Chaperone Hsp70 antibody
      • Chaperone protein dnaK antibody
      • Co chaperone with DnaJ antibody
      • dnaK antibody
      • Heat shock 70 kDa protein antibody
      • HSP70 antibody
      see all

    画像

    • Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
      Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
      All lanes :

      Lane 1 : DnaK active recombinant protein
      Lane 2 : E.coli cell lysate.

      Predicted band size: 70 kDa



      This image was generated using the ascites version of the product.

    • Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
      Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
      Anti-DnaK antibody [8E2/2] (ab69617) at 0.1 µg/ml + E.Coli DnaK protein

      Predicted band size: 70 kDa
      Observed band size: 70 kDa



      This image was generated using the ascites version of the product.

    プロトコール

    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (37)

    ab69617 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab69617 は 37 報の論文で使用されています。

    • Liu B  et al. Enterohaemorrhagic E. coli utilizes host- and microbiota-derived L-malate as a signaling molecule for intestinal colonization. Nat Commun 14:7227 (2023). PubMed: 37945607
    • Branton WG  et al. Microbial molecule ingress promotes neuroinflammation and brain CCR5 expression in persons with HIV-associated neurocognitive disorders. Brain Behav Immun 107:110-123 (2023). PubMed: 36202168
    • Frumkin I & Laub MT Selection of a de novo gene that can promote survival of Escherichia coli by modulating protein homeostasis pathways. Nat Ecol Evol 7:2067-2079 (2023). PubMed: 37945946
    • Liu J  et al. Mechanistic insights into the subversion of the linear ubiquitin chain assembly complex by the E3 ligase IpaH1.4 of Shigella flexneri. Proc Natl Acad Sci U S A 119:e2116776119 (2022). PubMed: 35294289
    • Cohen H  et al. Intracellular Salmonella Paratyphi A is motile and differs in the expression of flagella-chemotaxis, SPI-1 and carbon utilization pathways in comparison to intracellular S. Typhimurium. PLoS Pathog 18:e1010425 (2022). PubMed: 35381053
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-DnaK antibody [8E2/2]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Bacteria Cell lysate - whole cell (Exponential/stationary B. thetaiotaomicron 0.2 OD)
    Gel Running Conditions
    Reduced Denaturing (7% PAA tris acetate gel)
    Loading amount
    0.2 cells
    Specification
    Exponential/stationary B. thetaiotaomicron 0.2 OD
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 25 2019

    Western blot abreview for Anti-DnaK antibody [8E2/2]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Sodalis glossinidius Cell lysate - whole cell (bacteria)
    Loading amount
    30 µg
    Specification
    bacteria
    Treatment
    42C heatshock for 5 min
    Gel Running Conditions
    Reduced Denaturing (12.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Laura Runyen-Janecky

    Verified customer

    投稿 Mar 01 2013

    Question

    I am interested in purchasing and testing the Anti-DnaK antibody [8E2/2] (ab69617) to use in a Western Blot with the bacterium Sodalis glossinidius. Do you have any data on the cross-reactivity of the E. coli specific antibody with other bacterial species? The Sodlalis DnaK protein is 89% identical to the E. coli DnaK.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 26 2012

    Answer

    DISCOUNT CODE: ****
    Expiration date: March 27, 2013
    Value: $337

    We do not have data for that species but I am very pleased to hear you would like to accept our offer and test ab69617 on samples of Sodalis glossinidius. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Sodalis glossinidius and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    Answered on Nov 26 2012

    Question



    Thanks for the info. I’d be glad to test the antibody with our bacteria. However, I am a bit confused. How is this a discount if I pay full price to start? What if the antibody does not work?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 20 2012

    Answer

    Thank you for your reply.

    You are correct that you have to purchase the antibody in question first (at full price, but after you have submitted the Abreview on the antibody performed (and it can be positive or negative), you can use the code that was sent to you to receive the value that you paid for the first product off your next order.

    Please let me know if you would like me to issue the AbTrial code for you, or if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Nov 20 2012

    Question

    I am interested in purchasing the Anti-DnaK antibody [8E2/2] (ab69617) to use in a Western Blot with the bacterium Sodalis glossinidius. Do you have any data on the cross-reactivity of the E. coli specific antibody with other bacterial species? The Sodlalis DnaK protein is 89% identical to the E. coli DnaK

    Read More

    Abcam community

    Verified customer

    Asked on Nov 20 2012

    Answer

    Thank you for contacting Abcam
    .
    To our knowledge, ab69617 has not been tested in with other bacterial species, including the Sodlalis DnaK protein. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing this product.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    Answered on Nov 20 2012

    Question

    This customer would like to order ab69617 (Anti-DnaK antibody [8E2/2]), She would likes to order this antibody and test in wb with Xanthomonas campestris pv. Campestris (Xcc) sample, would you please help this customer to create a discount code?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 30 2011

    Answer

    DISCOUNT CODE: ******** Expiration date: DD MM YYYY I am very pleased to hear you would like to accept our offer and test ab69617 in Xanthomonas campestris pv. Campestris. The homology between e. coli DnaK and Xcc DnaK is 72.6% (see attached pdf for more details). This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for Xanthomonas campestris pv. Campestris and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Dec 30 2011

    Question

    Thanks for your kindly reply, after I contacted with this customer she provided the whole Xanthomonas campestris pv. Campestris (Xcc) sequence which I attached in this letter already, therefore, could you please help this customer to offer more information? Thanks for your kindly help. Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2011

    Answer

    Thank you and your customer for providing the sequence of DnaK from Xanthomonas campestris pv. Campestris. Both antibodies were generated with the full-length DnaK protein; ab69617 with E.coli DnaK and ab73473 with DnaK from Mycobacterium avium ssp. paratuberculosis. I aligned these sequences to the Xanthomonas campestris pv. Campestris sequence your customer provided und I have to confirm they only show a low homology (72% and 54%). We generally expect some cross-reactivity from 85% homology and therefore I cannot recommend these antibodies for Xanthomonas campestris pv. Campestris. I am sorry that I do not have a positive answer on this occasion and hope this information is nevertheless helpful. Please do not hesitate to contact me again with any further questions in this matter.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2011

    Question

    Dear technical support: This customer raised an enquire about ab69617 (Anti-DnaK antibody [8E2/2]) and ab73473 (Anti-DnaK antibody [7D9D7]), she wants to know whether these antibodies can recognize to the Danks protein which was secrete by Xanthomonas campestris pv. Campestris (Xcc) or not? If not, could you please offer any suitable selection to this customer? Thanks for your kindly help.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 19 2011

    Answer

    Thank you for your inquiry. Unfortunately, I do not have access to the sequence of DnaK of Xanthomonas campestris pv. Campestris. Could you please ask your customer to provide me with this sequence? I will then make an alignment. If the homology to the E.coli or Mycobacterium avium ssp. paratuberculosis protein is more than 85% I will be very happy to issue a testing discount code for your customer. I am looking forward to your answer.

    Read More

    Abcam Scientific Support

    Answered on Dec 19 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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