AB179830

Anti-DNAJC7 抗体 [EPR13349] - N-terminal

Anti-DNAJC7 antibody [EPR13349] - N-terminal

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal DNAJC7 antibody. N-terminal. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

別名を表示する

TPR2, TTC2, DNAJC7, DnaJ homolog subfamily C member 7, Tetratricopeptide repeat protein 2, TPR repeat protein 2

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with ab179830 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Supplier Data

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

All lanes:

Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 56 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13349

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF, Flow Cyt (Intra), IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Acts as a co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes. Recruits NR1I3 to the cytoplasm (By similarity).

See full target information DNAJC7

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111086 PubMed35858576

2022

Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Gao,Jinge Gu,Hong Zhang,Kai Jiang,Linlin Tang,Ren Liu,Li Zhang,Pengfei Zhang,Cong Liu,Bin Dai,Jie Song

Cell death discovery 8:208 PubMed35436989

2022

GBP2 facilitates the progression of glioma via regulation of KIF22/EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yeqing Ren,Biao Yang,Geng Guo,Jianping Zhang,Yanqi Sun,Dong Liu,Shihao Guo,Yongqiang Wu,Xiaogang Wang,Shule Wang,Wenju Zhang,Xiaolong Guo,Xuepeng Li,Ren Li,Jianhang He,Zihan Zhou

Cells 11: PubMed35053380

2022

Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Qin,Yu Xin,Yong Bian,Xuan Yang,Tao Xi,Jing Xiong

Nature neuroscience 22:1966-1974 PubMed31768050

2019

Exome sequencing in amyotrophic lateral sclerosis implicates a novel gene, DNAJC7, encoding a heat-shock protein.

Applications

Unspecified application

Species

Unspecified reactive species

Sali M K Farhan,Daniel P Howrigan,Liam E Abbott,Joseph R Klim,Simon D Topp,Andrea E Byrnes,Claire Churchhouse,Hemali Phatnani,Bradley N Smith,Evadnie Rampersaud,Gang Wu,Joanne Wuu,Aleksey Shatunov,Alfredo Iacoangeli,Ahmad Al Khleifat,Daniel A Mordes,Sulagna Ghosh,Kevin Eggan,Rosa Rademakers,Jacob L McCauley,Rebecca Schüle,Stephan Züchner,Michael Benatar,J Paul Taylor,Michael Nalls,Marc Gotkine,Pamela J Shaw,Karen E Morrison,Ammar Al-Chalabi,Bryan Traynor,Christopher E Shaw,David B Goldstein,Matthew B Harms,Mark J Daly,Benjamin M Neale

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNAJC7 抗体 [EPR13349] - N-terminal

Anti-DNAJC7 antibody [EPR13349] - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

関連する標識済み抗体及び組成の異なる製品 (10)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise