Anti-DNAJC3 抗体 [EP597Y] (ab52899)
![Western blot - Anti-DNAJC3 antibody [EP597Y] (ab52899)](https://www.abcam.co.jp/ps/products/52/ab52899/Images/ab52899-65373-anti-dnajc3-antibody-ep597y-western-blot.gif "Western blot - Anti-DNAJC3 antibody [EP597Y] (ab52899)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP597Y] to DNAJC3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DNAJC3 antibody [EP597Y]
DNAJC3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP597Y] to DNAJC3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human DNAJC3 aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- HeLa cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP597Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52899の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa). |
ターゲット情報
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機能
Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. -
組織特異性
Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels. -
配列類似性
Contains 1 J domain.
Contains 9 TPR repeats. -
ドメイン
The J domain mediates interaction with HSPA8. -
細胞内局在
Endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 5611 Human
- Omim: 601184 Human
- SwissProt: Q13217 Human
- Unigene: 59214 Human
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別名
- DnaJ (Hsp40) homolog subfamily C member 3 antibody
- DnaJ homolog subfamily C member 3 antibody
- DNAJC3 antibody
see all
画像
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Western blot - Anti-DNAJC3 antibody [EP597Y] (ab52899)Anti-DNAJC3 antibody [EP597Y] (ab52899) at 1/1000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted? -
![Anti-DNAJC3 antibody [EP597Y] (ab52899)](https://www.abcam.co.jp/ps/products/52/ab52899/Images/ab52899-2-benefits-of-recombinant-antibodies.png)
Anti-DNAJC3 antibody [EP597Y] (ab52899)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab52899 は 1 報の論文で使用されています。
- Bergmann L et al. Subcellular Localization and Mitotic Interactome Analyses Identify SIRT4 as a Centrosomally Localized and Microtubule Associated Protein. Cells 9:N/A (2020). PubMed: 32846968