Anti-DNAJB9 抗体 (ab118282)
Key features and details
- Goat polyclonal to DNAJB9
- Suitable for: WB, ICC, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DNAJB9 antibody
DNAJB9 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DNAJB9 -
由来種
Goat -
アプリケーション
適用あり: WB, ICC, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog, Pig, Chimpanzee
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免疫原
Synthetic peptide:
PDAEAKFREIAEAYE
with a Cysteine residue linker, corresponding to internal sequence amino acids 61-75 of Human DNAJB9 (NP_036460.1). -
ポジティブ･コントロール
- WB: Human liver and kidney lysates. Mouse liver lysate; ICC: A431 and U2OS cells; Flow Cyt (intra): A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab118282 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118282の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
1 hour primary incubation is recommended for this product. |
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| ICC |
Use a concentration of 10 µg/ml.
|
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| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC
Use a concentration of 10 µg/ml. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Acts as a co-chaperone with an Hsp70 protein. -
配列類似性
Contains 1 J domain. -
細胞内局在
Cytoplasm. Nucleus. Stress induces its translocation to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 614588 Cow
- Entrez Gene: 4189 Human
- Entrez Gene: 27362 Mouse
- Entrez Gene: 24908 Rat
- Omim: 602634 Human
- SwissProt: Q9UBS3 Human
- SwissProt: Q9QYI6 Mouse
- SwissProt: P97554 Rat
see all -
別名
- DKFZP564F1862 antibody
- DnaJ (Hsp40) homolog subfamily B member 9 antibody
- DnaJ homolog subfamily B member 9 antibody
see all
画像
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Western blot - Anti-DNAJB9 antibody (ab118282)All lanes : Anti-DNAJB9 antibody (ab118282) at 1 µg/ml
Lane 1 : Human liver lysate (in RIPA buffer)
Lane 2 : Human kidney lysate (in RIPA buffer)
Lane 3 : Mouse liver lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 26 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Immunocytochemistry - Anti-DNAJB9 antibody (ab118282)Immunocytochemistry analysis of paraformaldehyde fixed U2OS cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Immunocytochemistry - Anti-DNAJB9 antibody (ab118282)Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (ab118282)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) labeling DNAJB9 with ab118282. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab118282 は 4 報の論文で使用されています。
- Bestepe F et al. Deficiency of miR-409-3p improves myocardial neovascularization and function through modulation of DNAJB9/p38 MAPK signaling. Mol Ther Nucleic Acids 32:995-1009 (2023). PubMed: 37332476
- Rashid F et al. The ORF8 protein of SARS-CoV-2 induced endoplasmic reticulum stress and mediated immune evasion by antagonizing production of interferon beta. Virus Res 296:198350 (2021). PubMed: 33626380
- Yoneda A et al. Heat Shock Protein 47 Maintains Cancer Cell Growth by Inhibiting the Unfolded Protein Response Transducer IRE1a. Mol Cancer Res 18:847-858 (2020). PubMed: 32102897
- Liu Z et al. Reducing Smad3/ATF4 was essential for Sirt1 inhibiting ER stress-induced apoptosis in mice brown adipose tissue. Oncotarget 8:9267-9279 (2017). WB . PubMed: 28030827