AB118282

Anti-DNAJB9 抗体

Name: Anti-DNAJB9 抗体 (ab118282) | Abcam
Brand: Abcam Limited
SKU: AB118282
Price: 67000 JPY
Availability: InStock

Anti-DNAJB9 antibody

(1 Review)

(4 Publications)

Goat Polyclonal DNAJB9 antibody. Suitable for ICC, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DNAJB9 aa 50-100.

別名を表示する

MDG1, UNQ743/PRO1471, DNAJB9, DnaJ homolog subfamily B member 9, Endoplasmic reticulum DNA J domain-containing protein 4, Microvascular endothelial differentiation gene 1 protein, ER-resident protein ERdj4, ERdj4, Mdg-1

4 Images

Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (AB118282)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (AB118282)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) labeling DNAJB9 with ab118282. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (1 μg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor^® 488 secondary antibody.

Supplier Data

Immunocytochemistry - Anti-DNAJB9 antibody (AB118282)

Immunocytochemistry analysis of paraformaldehyde fixed U2OS cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL).

Supplier Data

Immunocytochemistry - Anti-DNAJB9 antibody (AB118282)

Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL).

Supplier Data

Western blot - Anti-DNAJB9 antibody (AB118282)

Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

All lanes:

Western blot - Anti-DNAJB9 antibody (ab118282) at 1 µg/mL

Lane 1:

Human liver lysate (in RIPA buffer) at 35 µg

Lane 2:

Human kidney lysate (in RIPA buffer) at 35 µg

Lane 3:

Mouse liver lysate (in RIPA buffer) at 35 µg

Predicted band size: 26 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC, WB, Flow Cyt (Intra)

applications

免疫原

Synthetic Peptide within Human DNAJB9 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UBS3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab118282 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins (PubMed : 18400946). In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP : HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1 (By similarity). Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production (By similarity).

See full target information DNAJB9

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 32:995-1009 PubMed37332476

2023

Deficiency of miR-409-3p improves myocardial neovascularization and function through modulation of DNAJB9/p38 MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Furkan Bestepe,Colette Fritsche,Kartik Lakhotiya,Carolyn E Niosi,George F Ghanem,Gregory L Martin,Ruma Pal-Ghosh,Dakota Becker-Greene,James Weston,Ivana Hollan,Ivar Risnes,Stein Erik Rynning,Liv Heidi Solheim,Mark W Feinberg,Robert M Blanton,Basak Icli

Virus research 296:198350 PubMed33626380

2021

The ORF8 protein of SARS-CoV-2 induced endoplasmic reticulum stress and mediated immune evasion by antagonizing production of interferon beta.

Applications

Unspecified application

Species

Unspecified reactive species

Farooq Rashid,Emmanuel Enoch Dzakah,Haiying Wang,Shixing Tang

Molecular cancer research : MCR 18:847-858 PubMed32102897

2020

Heat Shock Protein 47 Maintains Cancer Cell Growth by Inhibiting the Unfolded Protein Response Transducer IRE1α.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Yoneda,Kaori Sakai-Sawada,Kenjiro Minomi,Yasuaki Tamura

Oncotarget 8:9267-9279 PubMed28030827

2016

Reducing Smad3/ATF4 was essential for Sirt1 inhibiting ER stress-induced apoptosis in mice brown adipose tissue.

Applications

Species

Unspecified reactive species

Zhenjiang Liu,Huihui Gu,Lu Gan,Yatao Xu,Fei Feng,Muhammad Saeed,Chao Sun

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNAJB9 抗体

Anti-DNAJB9 antibody

Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (AB118282)

Immunocytochemistry - Anti-DNAJB9 antibody (AB118282)

Immunocytochemistry - Anti-DNAJB9 antibody (AB118282)

Western blot - Anti-DNAJB9 antibody (AB118282)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise