Anti-DNAJB12 抗体 (ab154410)
Key features and details
- Rabbit polyclonal to DNAJB12
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DNAJB12 antibody -
製品の詳細
Rabbit polyclonal to DNAJB12 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow
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免疫原
Recombinant fragment corresponding to a region within amino acids 82-353 of Human DNAJB12 (Uniprot ID Q9NXW2)
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ポジティブ･コントロール
- A549, H1299, HCT116 and MCF7 cell lysates; Human colon carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154410の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
ターゲット情報
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配列類似性
Contains 1 J domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 423707 Chicken
- Entrez Gene: 533603 Cow
- Entrez Gene: 54788 Human
- Entrez Gene: 56709 Mouse
- Entrez Gene: 294513 Rat
- Omim: 608376 Human
- SwissProt: Q58DR2 Cow
- SwissProt: Q9NXW2 Human
see all -
別名
- DJ10 antibody
- DJB12_HUMAN antibody
- DKFZp586B2023 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB12 antibody (ab154410)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, labeling DNAJB12 using ab154410 at a 1/500 dilution.
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- Anti-DNAJB12 antibody (ab154410)All lanes : Anti-DNAJB12 antibody (ab154410) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
10% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154410 は 1 報の論文で使用されています。
- Ma P et al. HNF1A-Induced lncRNA HCG18 Facilitates Gastric Cancer Progression by Upregulating DNAJB12 via miR-152-3p. Onco Targets Ther 13:7641-7652 (2020). PubMed: 32801777