Anti-DNA Polymerase Kappa/POLK 抗体 (ab86076)
Key features and details
- Rabbit polyclonal to DNA Polymerase Kappa/POLK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DNA Polymerase Kappa/POLK antibody
DNA Polymerase Kappa/POLK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DNA Polymerase Kappa/POLK -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human DNA Polymerase Kappa/POLK aa 650-750.
Database link: Q9UBT6 -
ポジティブ･コントロール
- WB: HeLa or 293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86076の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 99 kDa. |
ターゲット情報
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機能
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. -
組織特異性
Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes. -
配列類似性
Belongs to the DNA polymerase type-Y family.
Contains 2 UBZ-type zinc fingers.
Contains 1 umuC domain. -
ドメイン
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. -
細胞内局在
Nucleus. Detected throughout the nucleus and at replication foci. - Information by UniProt
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参照データベース
- Entrez Gene: 51426 Human
- Omim: 605650 Human
- SwissProt: Q9UBT6 Human
- Unigene: 726312 Human
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別名
- DinB homolog 1 (E. coli) antibody
- DINB protein antibody
- DINB1 antibody
see all
画像
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Western blot - Anti-DNA Polymerase Kappa/POLK antibody (ab86076)All lanes : Anti-DNA Polymerase Kappa/POLK antibody (ab86076) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 99 kDa
Detection: chemiluminescence with exposure time of 3 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab86076 は論文での使用が確認できていません。