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Anti-DNA Polymerase Kappa/POLK 抗体 (ab115625)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase Kappa/POLK antibody (ab115625)

    Key features and details

    • Goat polyclonal to DNA Polymerase Kappa/POLK
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-DNA Polymerase Kappa/POLK antibody
      DNA Polymerase Kappa/POLK 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to DNA Polymerase Kappa/POLK

    • 由来種

      Goat

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse

    • 免疫原

      Synthetic peptide:

      PVNQPKESSRSTGS

      corresponding to amino acids 817-830 of Human DNA Polymerase Kappa/POLK, conjugated to KLH.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Human prostate tissue

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab115625の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

    • 組織特異性

      Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.

    • 配列類似性

      Belongs to the DNA polymerase type-Y family.
      Contains 2 UBZ-type zinc fingers.
      Contains 1 umuC domain.

    • ドメイン

      The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.

    • 細胞内局在

      Nucleus. Detected throughout the nucleus and at replication foci.
    • Information by UniProt

    • 参照データベース

      • 別名

        • DinB homolog 1 (E. coli) antibody
        • DINB protein antibody
        • DINB1 antibody
        • DINP antibody
        • DNA damage inducible protein b antibody
        • DNA polymerase kappa antibody
        • Polk antibody
        • POLK_HUMAN antibody
        • Polymerase (DNA directed) kappa antibody
        • polymerase, DNA, kappa antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase Kappa/POLK antibody (ab115625)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase Kappa/POLK antibody (ab115625)
        ab115625, at 5 µg/ml, staining DNA Polymerase Kappa/POLK in formalin fixed, paraffin embedded Human prostate tissue by Immunohistochemistry. Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

      参考文献 (0)

      ab115625 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab115625 は論文での使用が確認できていません。

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