Anti-DNA Polymerase beta 抗体 (ab26343)
Key features and details
- Rabbit polyclonal to DNA Polymerase beta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DNA Polymerase beta antibody
DNA Polymerase beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DNA Polymerase beta -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Dog, Xenopus laevis -
免疫原
Synthetic peptide corresponding to Human DNA Polymerase beta aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab26343の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (5) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P | (1) |
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
配列類似性
Belongs to the DNA polymerase type-X family. -
ドメイン
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity. -
翻訳後修飾
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. -
細胞内局在
Nucleus, Cytoplasm - Information by UniProt
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参照データベース
- Entrez Gene: 426794 Chicken
- Entrez Gene: 5423 Human
- Entrez Gene: 18970 Mouse
- Entrez Gene: 29240 Rat
- Entrez Gene: 397973 Xenopus laevis
- Omim: 174760 Human
- SwissProt: P06746 Human
- SwissProt: Q8K409 Mouse
see all -
別名
- DNA directed DNA polymerase beta antibody
- DNA pol beta antibody
- DNA polymerase beta antibody
see all
画像
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All lanes : Anti-DNA Polymerase beta antibody (ab26343) at 0.5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 150 kDa (possible cross reactivity), 48 kDa (possible cross reactivity), 90 kDa (possible cross reactivity) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody (ab26343)
Formaldehyde-fixed human small cell lung cancer tissue stained for DNA Polymerase beta using ab26343 at 1/200 dilution in immunohistochemical analysis.
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All lanes : Anti-DNA Polymerase beta antibody (ab26343) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 120 kDa (possible cross reactivity), 30 kDa (possible cross reactivity) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody (ab26343)Image courtesy of Human Protein Atlas
Image courtesy of Human Protein Atlas ab26343 staining DNA polymerase beta in Human gall bladder, showing a strong nuclear staining pattern in the columnar epithelial cells in contrast with the cells in the lamina propria. Paraffin embedded human gall bladder tissue was incubated with ab26343 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab26343 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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ICC/IF image of ab26343 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26343 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (44)
ab26343 は 44 報の論文で使用されています。
- Hao Q et al. DNA repair mechanisms that promote insertion-deletion events during immunoglobulin gene diversification. Sci Immunol 8:eade1167 (2023). PubMed: 36961908
- Alblihy A et al. Targeting Mre11 overcomes platinum resistance and induces synthetic lethality in XRCC1 deficient epithelial ovarian cancers. NPJ Precis Oncol 6:51 (2022). PubMed: 35853939
- Spanjaard A et al. Huwe1 supports B-cell development, B-cell-dependent immunity, somatic hypermutation and class switch recombination by regulating proliferation. Front Immunol 13:986863 (2022). PubMed: 36700204
- Iacono D et al. Reduction of pTau and APP levels in mammalian brain after low-dose radiation. Sci Rep 11:2215 (2021). PubMed: 33500491
- Al-Kawaz A et al. The frequency and clinical significance of DNA polymerase beta (POLβ) expression in breast ductal carcinoma in situ (DCIS). Breast Cancer Res Treat 190:39-51 (2021). PubMed: 34406589