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リコンビナントRabMAb

Anti-DNA PKcs (phospho S2056) 抗体 [EPR5670] - BSA and Azide free (ab174576)

Submit a review Submit a question References (2)
ChIC/CUT&RUN sequencing - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5670] to DNA PKcs (phospho S2056) - BSA and Azide free
  • Suitable for: IHC-P, WB, ELISA, ICC/IF, ChIC/CUT&RUN-seq
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free
    DNA PKcs 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5670] to DNA PKcs (phospho S2056) - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, ELISA, ICC/IF, ChIC/CUT&RUN-seqmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat whole cell lysate (ab7899). IHC-P: Human lung carcinoma and brain tissues. ICC/IF: Jurkat cells. ChIC/CUT&RUN-Seq: U2OS cells.

  • 特記事項

    ab174576 is the carrier-free version of ab124918.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab174576の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 469 kDa.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 469 kDa.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.

  • 配列類似性

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
    Contains 3 TPR repeats.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
    S-nitrosylated by GAPDH.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DNA dependent protein kinase catalytic subunit antibody
      • DNA PK catalytic subunit antibody
      • DNA-dependent protein kinase catalytic subunit antibody
      • DNA-PK catalytic subunit antibody
      • DNA-PKcs antibody
      • DNAPK antibody
      • DNAPK catalytic subunit antibody
      • DNPK 1 antibody
      • DNPK1 antibody
      • Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
      • Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody
      • HYRC 1 antibody
      • HYRC antibody
      • HYRC1 antibody
      • IMD26 antibody
      • p350 antibody
      • p460 antibody
      • PKRDC antibody
      • PRKDC antibody
      • PRKDC_HUMAN antibody
      • Protein Kinase DNA Activated Catalytic Polypeptide antibody
      • XRCC 7 antibody
      • XRCC7 antibody
      see all

    画像

    • ChIC/CUT&RUN sequencing - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      ChIC/CUT&RUN sequencing - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/µL, 2.5 x 10^5 U2OS cells treated with Etoposide (10μM 20h) and 5 µg of ab124918 [EPR5670]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

      Additional screenshots of mapped reads can be downloaded here.

      The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      Immunocytochemistry/Immunofluorescence analysis of Jurkat cells (untreated and treated with Alkaline Phosphatase) labelling DNA PKcs (phospho S2056) with purified ab124918 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DNA PKcs (phospho S2056) with purified ab124918 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling DNA PKcs (phospho S2056) with unpurified ab124918 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      Serially diluted ab124918 was bound to immobilised phospho- or control peptides (1 microgram per mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

    参考文献 (2)

    ab174576 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab174576 は 2 報の論文で使用されています。

    • Xie Y  et al. RBX1 prompts degradation of EXO1 to limit the homologous recombination pathway of DNA double-strand break repair in G1 phase. Cell Death Differ 27:1383-1397 (2020). PubMed: 31562368
    • El Bezawy R  et al. miR-205 enhances radiation sensitivity of prostate cancer cells by impairing DNA damage repair through PKCe and ZEB1 inhibition. J Exp Clin Cancer Res 38:51 (2019). PubMed: 30717752

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