Anti-DNA PKcs 抗体 (ab70250)
Key features and details
- Rabbit polyclonal to DNA PKcs
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DNA PKcs antibody
DNA PKcs 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DNA PKcs -
由来種
Rabbit -
特異性
Ku-80 co-immunoprecipitates using this antibody. -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide corresponding to Human DNA PKcs (C terminal). Immunogen is in the region of aa 4075 and the c terminus.
Database link: P78527 -
ポジティブ･コントロール
- Whole cell lysate from Hela cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70250の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 469 kDa (predicted molecular weight: 469 kDa).
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| IP |
Use at an assay dependent concentration.
Use at 2 to 5 µg/mg lysate. |
| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 469 kDa (predicted molecular weight: 469 kDa). |
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IP
Use at an assay dependent concentration. Use at 2 to 5 µg/mg lysate. |
ターゲット情報
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機能
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. -
配列類似性
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.
Contains 3 TPR repeats. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
S-nitrosylated by GAPDH. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5591 Human
- Entrez Gene: 19090 Mouse
- Entrez Gene: 360748 Rat
- Omim: 600899 Human
- SwissProt: P78527 Human
- SwissProt: P97313 Mouse
- Unigene: 491682 Human
- Unigene: 71 Mouse
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別名
- DNA dependent protein kinase catalytic subunit antibody
- DNA PK catalytic subunit antibody
- DNA-dependent protein kinase catalytic subunit antibody
see all
画像
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Western blot - Anti-DNA PKcs antibody (ab70250)All lanes : Anti-DNA PKcs antibody (ab70250) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 15 µg
Lane 2 : Whole cell lysate from Hela cells at 50 µg
Predicted band size: 469 kDa
Observed band size: 469 kDa
Additional bands at: 170 kDa, 180 kDa, 230 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-DNA PKcs antibody (ab70250)Immunoprecipitation/ Western Blot of DNA PKcs
Lane 1: ab70250 at 3µg/100µg for IP of HeLa whole cell lysate (1mg) followed by ab70250 at 0.1µg/ml for WB.
Lane 2: Control IgG.
Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab70250 は 26 報の論文で使用されています。
- Bass TE et al. ATM Regulation of the Cohesin Complex Is Required for Repression of DNA Replication and Transcription in the Vicinity of DNA Double-Strand Breaks. Mol Cancer Res 21:261-273 (2023). PubMed: 36469004
- Sutcu HH et al. DNA-PKcs regulates myogenesis in an Akt-dependent manner independent of induced DNA damage. Cell Death Differ 30:1900-1915 (2023). PubMed: 37400716
- Zhang Q et al. APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress. J Transl Med 21:183 (2023). PubMed: 36894994
- Unfried JP et al. Long Noncoding RNA NIHCOLE Promotes Ligation Efficiency of DNA Double-Strand Breaks in Hepatocellular Carcinoma. Cancer Res 81:4910-4925 (2021). PubMed: 34321241
- Wright TC et al. Abiraterone In Vitro Is Superior to Enzalutamide in Response to Ionizing Radiation. Front Oncol 11:700543 (2021). PubMed: 34367984