Anti-DNA PKcs 抗体 [18-2] (ab44815)
![Western blot - Anti-DNA PKcs antibody [18-2] (ab44815)](https://www.abcam.co.jp/ps/products/44/ab44815/Images/ab44815-268428-anti-dna-pkcs-antibody-18-2-western-blot.jpg "Western blot - Anti-DNA PKcs antibody [18-2] (ab44815)")
Key features and details
- Mouse monoclonal [18-2] to DNA PKcs
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DNA PKcs antibody [18-2]
DNA PKcs 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [18-2] to DNA PKcs -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Other Immunogen Type corresponding to Human DNA PKcs aa 1-2713. Immunogen is purified from HeLa cells.
Database link: P78527 -
エピトープ
amino acids 1-2713 -
ポジティブ･コントロール
- WB: Wildtype HAP1, SHSY5Y and K562 cell lysates. ICC/IF: Wildtype HAP1 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
18-2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab44815の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100.
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| WB | (1) |
1/200. Predicted molecular weight: 469 kDa.
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| 特記事項 |
|---|
|
ICC/IF
1/100. |
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WB
1/200. Predicted molecular weight: 469 kDa. |
ターゲット情報
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機能
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. -
配列類似性
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.
Contains 3 TPR repeats. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
S-nitrosylated by GAPDH. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5591 Human
- Omim: 600899 Human
- SwissProt: P78527 Human
- Unigene: 491682 Human
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別名
- DNA dependent protein kinase catalytic subunit antibody
- DNA PK catalytic subunit antibody
- DNA-dependent protein kinase catalytic subunit antibody
see all
画像
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Western blot - Anti-DNA PKcs antibody [18-2] (ab44815)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: DNA PKcs knockout HAP1 cell lysate (40 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: SHSY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab44815 observed at 460 kDa. Red - loading control, ab18251, observed at 52 kDa.ab44815 was shown to specifically react with DNA PKcs in iwld-type HAP1 cells. No band was observed when DNA PKcs knockout samples were examined. Wild-type and DNA PKcs knockout samples were subjected to SDS-PAGE. Ab44815 and ab18251 (loading control to alpha tubulin) were diluted at 1/200 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs antibody [18-2] (ab44815)](https://www.abcam.co.jp/ps/products/44/ab44815/Images/ab44815-281493-anti-dna-pkcs-antibody-18-2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs antibody [18-2] (ab44815)ab44815 staining DNA PKcs in wild-type HAP1 cells (top panel) and PRKDC knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab44815 at 1/100 dilution and ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab44815 は 11 報の論文で使用されています。
- Lal S et al. Discovery and Characterization of ZL-2201, a Potent, Highly Selective, and Orally Bioavailable Small-molecule DNA-PK Inhibitor. Cancer Res Commun 3:1731-1742 (2023). PubMed: 37663435
- Wu WY et al. SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway. Redox Biol 52:102303 (2022). PubMed: 35390676
- van de Kooij B et al. Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks. Nat Commun 13:5295 (2022). PubMed: 36075911
- Zeng X et al. MAPK4 silencing together with a PARP1 inhibitor as a combination therapy in triple‑negative breast cancer cells. Mol Med Rep 24:N/A (2021). PubMed: 34080025
- Luo Y et al. Enzalutamide-Resistant Progression of Castration-Resistant Prostate Cancer Is Driven via the JAK2/STAT1-Dependent Pathway. Front Mol Biosci 8:652443 (2021). PubMed: 34746227