Anti-DNA Ligase III/LIG3 抗体 (ab185815)
Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DNA Ligase III/LIG3 antibody
DNA Ligase III/LIG3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DNA Ligase III/LIG3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human DNA Ligase III/LIG3 (C terminal).
Database link: P49916 -
ポジティブ･コントロール
- WB: BT474, SW480, HeLa, HT-29, Jurkat IP: Jurkat
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185815の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500 - 1/2000.
|
| IP |
1/50 - 1/200.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/2000. |
|
IP
1/50 - 1/200. |
ターゲット情報
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機能
Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. -
組織特異性
Testis, thymus, prostate and heart. -
配列類似性
Belongs to the ATP-dependent DNA ligase family.
Contains 1 BRCT domain.
Contains 1 PARP-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3980 Human
- Omim: 600940 Human
- SwissProt: P49916 Human
- Unigene: 100299 Human
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別名
- D11Wsu78e antibody
- DNA ligase antibody
- DNA ligase 3 antibody
see all
画像
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Western blot - Anti-DNA Ligase III/LIG3 antibody (ab185815)All lanes : Anti-DNA Ligase III/LIG3 antibody (ab185815) at 1/1000 dilution
Lane 1 : BT474
Lane 2 : SW480
Lane 3 : HeLa
Lane 4 : HT-29
Lane 5 : Jurkat
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Observed band size: 113 kDa why is the actual band size different from the predicted?Blocking buffer: 3% nonfat dry milk in TBST
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Immunoprecipitation - Anti-DNA Ligase III/LIG3 antibody (ab185815)DNA Ligase III/LIG3 was immunoprecipitated from 200μg Jurkat cells using 1μg ab185815. Western blot was performed on the immunoprecipitate using ab185815 at 1/1000 dilution.
Lane 1: Input (Jurkat cell lysate)
Lane 2: Control IgG
Lane 3: ab185815 IP in Jurkat cell lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab185815 は 6 報の論文で使用されています。
- Batenburg NL et al. CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin. Int J Mol Sci 24:N/A (2023). PubMed: 37569794
- Gelot C et al. Polθ is phosphorylated by PLK1 to repair double-strand breaks in mitosis. Nature 621:415-422 (2023). PubMed: 37674080
- Li M et al. circCIMT Silencing Promotes Cadmium-Induced Malignant Transformation of Lung Epithelial Cells Through the DNA Base Excision Repair Pathway. Adv Sci (Weinh) 10:e2206896 (2023). PubMed: 36814305
- Lototska L et al. Human RAP1 specifically protects telomeres of senescent cells from DNA damage. EMBO Rep 21:e49076 (2020). PubMed: 32096305
- Hu HL et al. TOP2ß-Dependent Nuclear DNA Damage Shapes Extracellular Growth Factor Responses via Dynamic AKT Phosphorylation to Control Virus Latency. Mol Cell 74:466-480.e4 (2019). PubMed: 30930055
- Galanos P et al. Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability. Genome Biol 19:37 (2018). PubMed: 29548335